利用不同产地分离的微生物生产果胶酶的研究

R. Rani, Adity Gupta, Sukhminderjit Kaur
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引用次数: 0

摘要

本研究旨在研究细菌种类中果胶酶的产生。采用汉金培养基对柑橘废弃物和土壤样品进行细菌分离。形态学和生化鉴定是从纯化的培养物中进行的,在汉金培养基上显示出清晰的区域。选择产生果胶酶最高的芽孢杆菌sp.2进行游离和固定化细胞状态的酶活性比较。经过48 h和72 h的培养,游离细胞状态下的酶活性比固定细胞状态下的酶活性有所增加。然而,经过96 h的培养,固定化细胞条件下的果胶酶产量高于游离细胞状态。农业工业废弃物如柑橘类水果的果皮、腐烂的蔬菜废弃物、糖蜜等可用于分离产生果胶酶的细菌
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Studies on pectinase production using microorganisms isolated from different fields
The present study was planned to examine the pectinase production in bacterial species. Citrus wastes and soil samples were processed for the isolation of bacterial species using Hankin's medium. Morphological and biochemical characterization was carried out from purified cultures that showed clear zones in the Hankin's medium plate. The highest pectinase producing Bacillus sp.2 was selected for comparison of enzyme activity between the free and immobilized cell states. Increased enzyme activity was observed in free cell state as compared to the immobilized cell condition after 48 h and 72 h of incubation. However, pectinase production was higher in immobilized cell condition as compared to the free cell state after 96 h of incubation. Agroindustrial waste such as peel of citrus fruits, rotten vegetable wastes, molasses, etc. could be explored for isolating pectinase producing bacteria
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