蛇耳的植物化学筛选及体内镇痛活性研究木板。

Labony Khandokar, Sanjida Rahman Mim, Rasel Rana
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引用次数: 1

摘要

水仙(山楂)木板。是孟加拉国的一种本土草药。本研究旨在探讨该植物在体内的化学成分和镇痛潜能。通过建立的化学试验,对芭芭拉甲醇提取物及其不同组分进行植物化学评价,以确定植物内次生代谢物的可用库。最后,证实了至少11类植物成分的存在,即生物碱、黄酮类、酚类、苷类、皂素、淀粉、植物甾醇、香豆素、单宁、树脂和醌类。其中,芭芭拉乙酸乙酯可溶性部位次生代谢产物种类最多。采用尾浸法和醋酸扭体法分别研究了粗甲醇提取物(MEAB)及其乙酸乙酯可溶部位(EAAB)对瑞士白化病小鼠中枢和外周伤害感觉的抑制作用。尾浸试验第90分钟,MEAB和EAAB分别以250和500 mg/kg体重(MEAB1、MEAB2、EAAB1、EAAB2)剂量给药,疼痛反应的延长率分别为240.41%、285.34%、302.83%和339.60%,而标准的戊唑嗪在相同时间点的延长率为421.36%。此外,在相同剂量下,实验样品对醋酸引起的小鼠扭体反应的疼痛感觉分别减少了66.81%,74.08%,76.81%和83.52%。在两种剂量下,EAAB的中枢和外周镇痛电位均明显高于MEAB。该研究结果首次在合适的动物模型上显示了该植物的药理潜力,为今后对该植物的科学研究提供了有效的基础。孟加拉药学杂志24(2):117-124,2021
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phytochemical Screening and In vivo Analgesic Activity of Ampelocissus barbata (Wall.) Planch.
Ampelocissus barbata (Wall.) Planch. is a native medicinal herb in Bangladesh. The present study was aimed at exploring the phytochemical compositions and analgesic potential of the plant in vivo. The methanol extract of A. barbata along with its different fractions were subjected to phytochemical evaluation through established chemical tests for ascertaining the available pool of secondary metabolites within the plant. Eventually, the presence of at least eleven classes of phytoconstituents viz. alkaloids, flavonoids, phenolics, glycosides, saponins, starch, phytosterols, coumarins, tanins, resins and quinones were demonstrated. Among the four partitonates, ethyl acetate soluble fraction of A. barbata illustrated the maximum types of secondary metabolites qualitatively. The ability of the crude methanol extract (MEAB) and its ethyl acetate soluble fraction (EAAB) to suppress nociception both centrally and peripherally were investigated in Swiss albino mice by tail immersion method and acetic acid-induced writhing method, respectively. At the 90th minute of the tail immersion study, both MEAB and EAAB administered at the doses of 250 and 500 mg/kg body weight (MEAB1, MEAB2, EAAB1, EAAB2) exhibited 240.41%, 285.34%, 302.83% and 339.60% elongation of pain response, respectively, in comparison to the standard, pentazocine which effectuated 421.36% elongation at the same mark. Moreover, the test samples at the same doses as before exhibited reduction of pain sensation by 66.81%, 74.08%, 76.81%, and 83.52%, respectively, in the acetic acid-induced writhing response in mice. Both the central and peripheral analgesic potentials of EAAB were discernibly higher than those of MEAB at both doses. The findings of the study represents the pharmacological potential of this species in appropriate animal model for the first time and provides valid foundation to warrant future scientific endeavors into the plant. Bangladesh Pharmaceutical Journal 24(2): 117-124, 2021
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