两种罕见血清型副溶血性弧菌混合感染致食物中毒1例病因分析

中国热带医学 Pub Date : 2020-11-01 DOI:10.13604/J.CNKI.46-1064/R.2020.11.21

Huang Xian, He Dongmei, Li Zhencui, Liang Yongqing, Lin Siling

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Eight strains of Vibrio parahaemolyticus were detected in 9 rectal swabs, with ratios of the blood classification type O2:K28 was 25.00% (2/8), and O8: K21 was: 75.00%(6 / 8); The similarity of O2∶K28 serotypes (VP18171 and VP18175) was 66.7% by PFGE molecular typing. The similarity of O8∶K21 serotype (VP18172, VP18173, VP18176) was 100.0%, and the similarity of O8∶K21 serotype (VP18177, VP18174, VP18170) was 88.4%-95.2%. The results of the drug sensitivity test showed that while 1 strain was resis⁃ tant to ampicillin and cefazolin, 1 strain was resistant to ampicillin and 3 strains were resistant to cefazolin, the other strains were sensitive to all of the tested drugs, and there was no multi-drug resistance. Conclusion The food poisoning was caused by mixed infection of two different serotypes O2∶K28 and O8∶K21 of Vibrio parahaemolyticus. 摘要：目的分析一起由两种罕见血清型混合感染引起的副溶血性弧菌食物中毒病原学的检测结果, 为食物中毒病原学检查途径提供依据。方法对一起在龙门县人民医院就诊食物中毒事件的 9 例患者采集其肛拭子样本, 参照《食品微生物学检验副溶血性弧菌检验》( GB 4789.7—2013) 等标准进行病原学分析鉴定。结果本次事件出现临床症状者 9 例, 其中≤20 岁 2 例, 20~<40 岁 1 例, 40~<60 岁 4 例, ≥60 岁 2 例; 9 份肛拭子样本检出 8 株副溶血性弧菌, 血清分型 O2: K28 构成比占 25.00% (2 / 8); O8: K21 构成比占 75.00%(6 / 8); 经 PFGE 分子分型 , 2 株 O2∶K28 血清型 (VP18171、VP18175) 相似度为 66.7%; 3 株 O8∶K21 血清型 (VP18172、VP18173、VP18176) 相似度为 100.0%, 与另外 3 株 O8∶K21 血清型 (VP18177、VP18174、VP18170) 相似度为 88.4%~95.2%; 药敏试验结果显示, 除有 1 株菌株对氨苄西林、头孢唑林二重耐药、1 株菌株对氨苄西林耐药、3 株菌株对头孢唑林耐药外, 其它菌株对所有测试药物均敏感, 无多重耐药现象。结论此次食物中毒是由 O2:K28 和 O8:K21 这两种罕见血清型的副溶血性弧菌混合感染引起的。","PeriodicalId":10045,"journal":{"name":"中国热带医学","volume":"20 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Etiological analysis of a case of food poisoning caused by mixed infection with two rare serotypes of Vibrio parahaemolyticus\",\"authors\":\"Huang Xian, He Dongmei, Li Zhencui, Liang Yongqing, Lin Siling\",\"doi\":\"10.13604/J.CNKI.46-1064/R.2020.11.21\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective To analyze the etiology of a food poisoning caused by mixed infection with two rare serotypes of Vibrio parahaemolyticus, and we provide the basis for the pathogen examination of food poisoning. 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引用次数: 0

摘要

目的分析一起罕见的两种血清型副溶血性弧菌混合感染引起的食物中毒的病因，为食物中毒的病原检查提供依据。方法根据GB 4789.7-2013《食品微生物学检验副溶血性弧菌》等标准，选取龙门县人民医院收治的9例食物中毒患者作为研究对象，收集其直肠拭子进行分析鉴定。结果共有9例患者出现临床症状，其中2例年龄≤20岁，1例年龄21 ~ <40岁，4例年龄41 ~ <60岁，2例年龄≥60岁。9份直肠拭子检出8株副溶血性弧菌，血分型O2:K28比例为25.00% (2/8)，O8: K21比例为75.00%(6 /8);PFGE分子分型O2∶K28血清型(VP18171和VP18175)的相似性为66.7%。O8∶K21血清型(VP18172、VP18173、VP18176)的相似性为100.0%，O8∶K21血清型(VP18177、VP18174、VP18170)的相似性为88.4% ~ 95.2%。药敏试验结果显示，1株对氨苄西林和头孢唑林均有抗性，1株对氨苄西林耐药，3株对头孢唑林耐药，其余菌株对所有试验药物均敏感，无多重耐药现象。结论副溶血性弧菌O2∶K28和O8∶K21两种不同血清型混合感染引起食物中毒。摘要：目的分析一起由两种罕见血清型混合感染引起的副溶血性弧菌食物中毒病原学的检测结果, 为食物中毒病原学检查途径提供依据。方法对一起在龙门县人民医院就诊食物中毒事件的9例患者采集其肛拭子样本,参照“食品微生物学检验副溶血性弧菌检验”(GB -2013 - 4789.7)等标准进行病原学分析鉴定。结果本次事件出现临床症状者 9 例, 其中≤20 岁 2 例, 20~<40 岁 1 例, 40~<60 岁 4 例, ≥60 岁 2 例; 9份肛拭子样本检出8株副溶血性弧菌,血清分型O2: K28构成比占25.00% (2/8);O8: k21，己烷(6 / 8);经但是脉冲场凝胶电泳的出现分子分型,2株O2∶K28血清型(VP18171 VP18175)相似度为66.7%;3株O8∶血K21清型(VP18172、VP18173 VP18176)相似度为100.0%,与另外3株O8∶血K21清型(VP18177、VP18174 VP18170)相似度为88.4% ~ 95.2%;药敏试验结果显示, 除有 1 株菌株对氨苄西林、头孢唑林二重耐药、1 株菌株对氨苄西林耐药、3 株菌株对头孢唑林耐药外, 其它菌株对所有测试药物均敏感, 无多重耐药现象。结论此次食物中毒是由O2: K28和O8: K21这两种罕见血清型的副溶血性弧菌混合感染引起的。

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Etiological analysis of a case of food poisoning caused by mixed infection with two rare serotypes of Vibrio parahaemolyticus

Objective To analyze the etiology of a food poisoning caused by mixed infection with two rare serotypes of Vibrio parahaemolyticus, and we provide the basis for the pathogen examination of food poisoning. Methods According to Food Microbiology Testing for Vibrio parahaemolyticus (GB 4789.7-2013) and other standards, 9 patients with food poisoning treated in Longmen County People’s Hospital were selected as research objects, and their rectal swabs were collected for analy⁃ sis and identification. Results There were a total of 9 patients with clinical symptoms, including 2 patients aged ≤20 years old, 1 patient aged 21-<40 years old , 4 patients aged 41-<60 years old , and 2 patients aged ≥60 years old. Eight strains of Vibrio parahaemolyticus were detected in 9 rectal swabs, with ratios of the blood classification type O2:K28 was 25.00% (2/8), and O8: K21 was: 75.00%(6 / 8); The similarity of O2∶K28 serotypes (VP18171 and VP18175) was 66.7% by PFGE molecular typing. The similarity of O8∶K21 serotype (VP18172, VP18173, VP18176) was 100.0%, and the similarity of O8∶K21 serotype (VP18177, VP18174, VP18170) was 88.4%-95.2%. The results of the drug sensitivity test showed that while 1 strain was resis⁃ tant to ampicillin and cefazolin, 1 strain was resistant to ampicillin and 3 strains were resistant to cefazolin, the other strains were sensitive to all of the tested drugs, and there was no multi-drug resistance. Conclusion The food poisoning was caused by mixed infection of two different serotypes O2∶K28 and O8∶K21 of Vibrio parahaemolyticus. 摘要：目的分析一起由两种罕见血清型混合感染引起的副溶血性弧菌食物中毒病原学的检测结果, 为食物中毒病原学检查途径提供依据。方法对一起在龙门县人民医院就诊食物中毒事件的 9 例患者采集其肛拭子样本, 参照《食品微生物学检验副溶血性弧菌检验》( GB 4789.7—2013) 等标准进行病原学分析鉴定。结果本次事件出现临床症状者 9 例, 其中≤20 岁 2 例, 20~<40 岁 1 例, 40~<60 岁 4 例, ≥60 岁 2 例; 9 份肛拭子样本检出 8 株副溶血性弧菌, 血清分型 O2: K28 构成比占 25.00% (2 / 8); O8: K21 构成比占 75.00%(6 / 8); 经 PFGE 分子分型 , 2 株 O2∶K28 血清型 (VP18171、VP18175) 相似度为 66.7%; 3 株 O8∶K21 血清型 (VP18172、VP18173、VP18176) 相似度为 100.0%, 与另外 3 株 O8∶K21 血清型 (VP18177、VP18174、VP18170) 相似度为 88.4%~95.2%; 药敏试验结果显示, 除有 1 株菌株对氨苄西林、头孢唑林二重耐药、1 株菌株对氨苄西林耐药、3 株菌株对头孢唑林耐药外, 其它菌株对所有测试药物均敏感, 无多重耐药现象。结论此次食物中毒是由 O2:K28 和 O8:K21 这两种罕见血清型的副溶血性弧菌混合感染引起的。

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来源期刊

中国热带医学

CiteScore

0.60

自引率

0.00%

发文量

13927

期刊介绍： China Tropical Medicine, was approved by the Ministry of Science and Technology in 2001, is the only tropical medicine periodical under the charge of the National Health Commission of China. It’s organized by Hainan Provincial Center for Disease Prevention and Control, and Chinese Preventive Medicine Association. The journal is indexed by the following database: Scopus database, Embase database, EBSCO Database, The Western Pacific Region index medicus (WPRIM), American Chemical Abstracts (CA), International Centre for Agricultural and Biological Sciences Research Database (CABI), Global Health Database, Database of the Ulrich's Periodicals Directory, China Science and Technology Core Journals, China Core Journals (Selection) Database, Database of Chinese Biomedical Literature, Comprehensive Evaluation Database of Chinese Academic Journals, CAJCD Code of Conduct Excellent Journal, Database of Chinese SCI-Tech Periodicals, China Journal Full Text Database.