Q3 Medicine
A. Kaloshin, A. Soldatenkova, E. M. Zimina, N. Mikhailova
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引用次数: 0

摘要

的目标。获得铜绿假单胞菌对实验性假单胞菌感染具有保护作用的融合重组蛋白。材料和方法。将铜绿假单胞菌基因oprF、oprl和弓形虫缺失型的融合序列克隆到质粒中,在大肠杆菌中表达。合成的重组蛋白在Ni-sepharose柱中纯化。小鼠腹腔注射重组蛋白两次,每隔2周评估其保护作用。免疫2周后腹腔注射铜绿假单胞菌PA103毒力培养物作为实验攻毒。结果:获得了3个融合重组蛋白;OprF-ΔOprl包含OprF蛋白全序列和OprI缺失变体(缺失前20个氨基酸);2. AOprF-AOprl由c端区(192 ~ 342个氨基酸)OprF和Oprl蛋白的缺失变体组成;3.。OprF- atox -ΔOprI包括OprF蛋白全序列、外毒素A无毒变异序列(不含106个c端氨基酸)和Oprl蛋白缺失变异序列。融合重组蛋白OprF-AOprl和OprF-aTox-ΔOprI分别在免疫剂量为25和50 pg时显示出最好的保护性能。结论。该结果为进一步研究以铜绿假单胞菌融合重组蛋白为基础制备特异性免疫生物制剂开辟了前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
OBTAINING FUSED RECOMBINANT PROTEINS OprF-ΔOprI, ΔOprF-ΔOprI AND OprF-aTox-ΔOprl OF PSEUDOMONAS AERUGINOSA
Aim. Obtaining fused recombinant proteins of Pseudomonas aeruginosa that have protective properties against experimental pseudomonas infection. Materials and methods. Fused sequences of P. aeruginosa genes oprF, oprl and deleted form of toxA were cloned in plasmids for the expression in Escherichia coli. The synthesized recombinant proteins were purified in Ni-sepharose columns. Recombinant proteins were administered to mice intraperitonealiy twice with a 2 week interval to evaluate protective properties. Virulent culture of P. aeruginosa strain PA103 was injected into the animals intraperitonealiy 2 weeks after the immunization course as experimental challenge. Results. 3 fused recombinant proteins were produced: 1. OprF-ΔOprl included full sequence of OprF protein and deletion variant of OprI (lacking first 20 amino acids); 2. AOprF-AOprl consisted of C-terminal region (192 - 342 amino acids) OprF and deletion variant of Oprl protein; 3. OprF-aTox-ΔOprI included full sequence of OprF protein, sequence of nontoxic variant of exotoxin A (without 106 C-terminal amino acids) and deletion variant of Oprl protein. Fused recombinant proteins OprF-AOprl and OprF-aTox-ΔOprI at immunization doses of 25 and 50 pg for the first and second protein, respectively, were shown to have the best protective properties. Conclusion. The results obtained open perspectives for further studies to create specific immune biological preparations based on fused recombinant proteins of P. aeruginosa.
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来源期刊
Zhurnal mikrobiologii, epidemiologii, i immunobiologii
Zhurnal mikrobiologii, epidemiologii, i immunobiologii Immunology and Microbiology-Immunology and Microbiology (miscellaneous)
CiteScore
1.40
自引率
0.00%
发文量
51
审稿时长
8 weeks
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