{"title":"一株高效薯蓣降解菌株的筛选","authors":"Yanjun Lu, Jifeng Guo","doi":"10.1109/ISWREP.2011.5893294","DOIUrl":null,"url":null,"abstract":"12 strains of cellulolytic microorganism were isolated from the AB reactor of dioscorea zingiberensis wastewater treatment. A bacterial strain was obtained through the screening experiments of decomposition of dioscorea zingiberensis, which had high degradability for dioscorea zingiberensis and was named D-1. The gene sequence obtained from the DNA extraction and polymerase chain reaction (PCR) amplification was compared with NCBI Genbank of U.S.A., and the strain was preliminary identified to be Clostridium cellulosolvens. The optimum growth conditions were pH 5.0, 30°C, 100 r/min.","PeriodicalId":6425,"journal":{"name":"2011 International Symposium on Water Resource and Environmental Protection","volume":"210 1","pages":"1438-1440"},"PeriodicalIF":0.0000,"publicationDate":"2011-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Screening a novel high effective dioscorea zingiberensis degradation strain\",\"authors\":\"Yanjun Lu, Jifeng Guo\",\"doi\":\"10.1109/ISWREP.2011.5893294\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"12 strains of cellulolytic microorganism were isolated from the AB reactor of dioscorea zingiberensis wastewater treatment. A bacterial strain was obtained through the screening experiments of decomposition of dioscorea zingiberensis, which had high degradability for dioscorea zingiberensis and was named D-1. The gene sequence obtained from the DNA extraction and polymerase chain reaction (PCR) amplification was compared with NCBI Genbank of U.S.A., and the strain was preliminary identified to be Clostridium cellulosolvens. The optimum growth conditions were pH 5.0, 30°C, 100 r/min.\",\"PeriodicalId\":6425,\"journal\":{\"name\":\"2011 International Symposium on Water Resource and Environmental Protection\",\"volume\":\"210 1\",\"pages\":\"1438-1440\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-05-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2011 International Symposium on Water Resource and Environmental Protection\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/ISWREP.2011.5893294\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2011 International Symposium on Water Resource and Environmental Protection","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/ISWREP.2011.5893294","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Screening a novel high effective dioscorea zingiberensis degradation strain
12 strains of cellulolytic microorganism were isolated from the AB reactor of dioscorea zingiberensis wastewater treatment. A bacterial strain was obtained through the screening experiments of decomposition of dioscorea zingiberensis, which had high degradability for dioscorea zingiberensis and was named D-1. The gene sequence obtained from the DNA extraction and polymerase chain reaction (PCR) amplification was compared with NCBI Genbank of U.S.A., and the strain was preliminary identified to be Clostridium cellulosolvens. The optimum growth conditions were pH 5.0, 30°C, 100 r/min.