Contribution of endothelium-derived relaxing factors to P2Y-purinoceptor-induced vasodilation in the isolated rat kidney

We examined the role of endothelium-derived relaxing factors nitric oxide (NO), endothelium-derived hyperpolarising factor (EDHF), and prostaglandins (PGs) to P_2Y1- and P_2Y2-purinoceptor-induced vasodilation in isolated rat kidney. To do it, we analysed the renal response to ATP, 2-methylthio ATP, and UTP in rat renal vasculature under normal conditions and after the administration of: N^w-nitro-l-arginine (l-NAME), increased K⁺ concentration, indomethacin, and l-NAME and increased K⁺ together. Our results indicate that the vasodilator response to P_2Y1- and P_2Y2-purinoceptor activation in the isolated perfused kidney of rats is subserved by EDHF and NO.