{"title":"日本鹌鹑催乳素基因的遗传多样性","authors":"F. Eichie","doi":"10.7439/IJBAR.V9I3.4602","DOIUrl":null,"url":null,"abstract":"This study was carried out to investigate the genetic diversity of prolactin gene (PRL gene) in Japanese quails using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) marker. One hundred and thirty nine quails were sampled for PRL loci analysis. DNA was extracted from the samples. Polymerase chain reaction (PCR) and electrophoresis was used to characterize a 24 base pair (bp) insertion/deletion in a 358 bp PCR product. Test for Hardy-Weinberg Equilibrium were carried out. From the results, two alleles A (0.63) and B (0.37) and three genotypes AA, AB and BB were observed from this locus. Results of the study showed that genetic variability exists in the study population of Japanese quails in Nigeria and its segregation could be assessed for reproductive capacity","PeriodicalId":13848,"journal":{"name":"International Journal of Biomedical and Advance Research","volume":"4 1","pages":"96-99"},"PeriodicalIF":0.0000,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Genetic diversity of Prolactin gene in Japanese quail (Coturnix Coturnix Japonica)\",\"authors\":\"F. Eichie\",\"doi\":\"10.7439/IJBAR.V9I3.4602\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study was carried out to investigate the genetic diversity of prolactin gene (PRL gene) in Japanese quails using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) marker. One hundred and thirty nine quails were sampled for PRL loci analysis. DNA was extracted from the samples. Polymerase chain reaction (PCR) and electrophoresis was used to characterize a 24 base pair (bp) insertion/deletion in a 358 bp PCR product. Test for Hardy-Weinberg Equilibrium were carried out. From the results, two alleles A (0.63) and B (0.37) and three genotypes AA, AB and BB were observed from this locus. Results of the study showed that genetic variability exists in the study population of Japanese quails in Nigeria and its segregation could be assessed for reproductive capacity\",\"PeriodicalId\":13848,\"journal\":{\"name\":\"International Journal of Biomedical and Advance Research\",\"volume\":\"4 1\",\"pages\":\"96-99\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Biomedical and Advance Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.7439/IJBAR.V9I3.4602\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biomedical and Advance Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.7439/IJBAR.V9I3.4602","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Genetic diversity of Prolactin gene in Japanese quail (Coturnix Coturnix Japonica)
This study was carried out to investigate the genetic diversity of prolactin gene (PRL gene) in Japanese quails using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) marker. One hundred and thirty nine quails were sampled for PRL loci analysis. DNA was extracted from the samples. Polymerase chain reaction (PCR) and electrophoresis was used to characterize a 24 base pair (bp) insertion/deletion in a 358 bp PCR product. Test for Hardy-Weinberg Equilibrium were carried out. From the results, two alleles A (0.63) and B (0.37) and three genotypes AA, AB and BB were observed from this locus. Results of the study showed that genetic variability exists in the study population of Japanese quails in Nigeria and its segregation could be assessed for reproductive capacity
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