水醇提取物降低A549肺癌细胞系中Hippo信号通路相关癌基因的表达

Current Chemical Biology Pub Date : 2023-02-14 DOI:10.2174/2212796817666230214100146

M. Ashtiani, Fariba Nabatchian, M. Davoudi, Negin Davoudi, Reza Afrisham

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引用次数: 0

摘要

本研究旨在评估该植物水酒精提取物对A549肺癌细胞株中Hippo信号通路相关癌基因和抑制基因mRNA表达的影响。有证据表明，在肺癌等少数疾病中，调节器官大小的河马通路发生了改变。在伊朗传统医学中用于治疗各种疾病的阿基里斯已经被证明有助于癌症的一些信号通路。因此，本研究旨在评价该植物水酒精提取物对A549肺癌细胞株中Hippo信号通路相关癌基因和抑制基因mRNA表达的影响。本研究旨在评估该植物水酒精提取物对A549肺癌细胞株中Hippo信号通路相关癌基因和抑制基因mRNA表达的影响。采用索氏提取法制备了水酒精提取物，并采用MTT法研究了其抗增殖活性。然后，利用实时荧光定量PCR检测大肿瘤抑制激酶1和2 (LATS1和LATS2)、Yes1相关转录调控因子(YAP1)和PDZ结合基序转录辅激活因子(TAZ)的mRNA表达。MTT结果显示，200 ~ 1000µg/ml的浓度处理24 h, 100 ~ 1000µg/ml的浓度处理48 h后，细胞活力显著降低。与未处理的细胞相比，LATS1、TAZ和YAP1的mRNA表达量显著降低，而LATS2的mRNA表达量没有变化(P小于0.05)。水醇提取物对肺癌细胞的增殖有抑制作用，并能降低肺癌细胞中两种致癌基因的表达。但其对LATS1有抑制作用，应在进一步研究中加以考虑

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Hydroalcoholic Extract of Achillea Wilhelmsii Decreases the Expressions of Hippo Signaling Pathway-Associated Oncogenes In the A549 Lung Cancer Cell Line

this study aimed to evaluate the effect of the hydroalcoholic extract of this plant on viability and mRNA expression of some Hippo signaling pathway-associated oncogenes and suppressors in A549 lung cancer cell lines. Evidence suggests that the Hippo pathway, which regulates organ size, is altered in a few conditions like lung cancer. Achillea wilhelmsii that used in the traditional Iranian medicine to treat a variety of disorders has been proven to contribute to some signaling pathways in cancers. In this regards, this study aimed to evaluate the effect of the hydroalcoholic extract of this plant on viability and mRNA expression of some Hippo signaling pathway-associated oncogenes and suppressors in A549 lung cancer cell lines. this study aimed to evaluate the effect of the hydroalcoholic extract of this plant on viability and mRNA expression of some Hippo signaling pathway-associated oncogenes and suppressors in A549 lung cancer cell lines. Hydroalcoholic extract was prepared using a Soxhlet extractor and its antiproliferative activity was studied by MTT assay. Then, the mRNA expressions of large tumour suppressor kinases 1 and 2 (LATS1 and LATS2), Yes1 Associated Transcriptional Regulator (YAP1), and Transcriptional co‑activator with PDZ‑binding motif (TAZ) were measured using real-time PCR. According to MTT, the viability was decreased significantly after 24 hours treatment with A. wilhelmsii at concentrations of 200-1000 µg/ml and after 48 hours treatment at a concentration of 100-1000 µg/ml. While the mRNA levels of LATS1, TAZ, and YAP1 decreased significantly compared to untreated cells, the mRNA expression of LATS2 did not change (P˂ 0.05). Hydroalcoholic extract of A. wilhelmsii might inhibit proliferation of lung cancer cells as well as it could decrease the expression of both oncogenes in them. However, it had suppressing effects on LATS1, which should be considered in further studies. none

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来源期刊

Current Chemical Biology Medicine-Biochemistry (medical)

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Current Chemical Biology aims to publish full-length and mini reviews on exciting new developments at the chemistry-biology interface, covering topics relating to Chemical Synthesis, Science at Chemistry-Biology Interface and Chemical Mechanisms of Biological Systems. Current Chemical Biology covers the following areas: Chemical Synthesis (Syntheses of biologically important macromolecules including proteins, polypeptides, oligonucleotides, oligosaccharides etc.; Asymmetric synthesis; Combinatorial synthesis; Diversity-oriented synthesis; Template-directed synthesis; Biomimetic synthesis; Solid phase biomolecular synthesis; Synthesis of small biomolecules: amino acids, peptides, lipids, carbohydrates and nucleosides; and Natural product synthesis).