分离和筛查由共生菌的NUDIBRANCHIA Phyllidiella pustulosa和Thuridilla lineolata提供的抗菌活性

Ade Kurniawan Arie, Rosita A J Lintang, R. Mangindaan, Agung B. Windarto, F. Losung, Sammy N. J. Longdong
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摘要

裸鳃动物是海洋无脊椎动物,属于腹足纲,能够伪装并发展出自卫系统。海洋细菌中含有的化学物质通过二次代谢对海洋药物的发现具有潜力。本研究的目的是从裸枝植物中分离出一株病原菌,并对其对大肠杆菌和金黄色葡萄球菌的抑菌活性进行筛选。在B1(固体)培养基上分离培养共生菌。抑菌试验结果表明,从脓疱芽孢杆菌和毛酸Thuridilla中分离出的共生细菌能够抑制金黄色葡萄球菌和大肠杆菌的生长。关键词:裸鳃目,脓包Phyllidiella pustulosa,细叶蕨,细菌,抗菌abstract摘要:裸鳃目,无脊椎目,无脊椎目,大鲵,腹足目,猕猴桃,猕猴桃,猕猴桃,猕猴桃,猕猴桃Bakteri laut mengandung senyawa kimia yang berpoteni sebagai bahan obatan yang dihasilkan melalui代谢sekunder。结核菌门鞭毛菌、细菌芽孢杆菌、抗细菌门鞭毛菌、分离细菌门鞭毛菌、大肠杆菌门鞭毛菌、金黄色葡萄球菌。从文化角度看,中国文化传播媒体B1 (padat)。拮抗细菌活性:杨氏细菌、白斑细菌、登根细菌、裸鳃细菌、脓疱杆菌、毛线霉、曼普、孟汉、pertumbuhan、微生物、金黄色葡萄球菌和大肠杆菌。Kata kunci:裸科,脓疱Phyllidiella pustulosa,毛线菌Thuridilla lineolate,巴克特菌,抗巴克特菌
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ISOLASI DAN SKRINING AKTIVITAS ANTIBAKTERI DARI BAKTERI SIMBION NUDIBRANCHIA Phyllidiella pustulosa dan Thuridilla lineolata
Nudibranchs are marine invetebrates belongs to the class of Gastropoda that are able to camouflage and develop a self-defense systems. Marine bacteria contain chemicals compunds that have potentials on marine drugs discovery through the secondary metabolism. The purpose of this study was to isolate the symbiotic bacteria from nudibranches Phylidiella pustulosa and Thuridilla lineolate and to screen the antibacterial activity of these bacterial isolates against Escherichia coli and Staphylococcus aureus. Isolation and culture of the symbiotic bacteria was made on B1 (solid) media. The results of the antibacterial assay showed that the symbiotic bacterial isolates from Phylidiella pustulosa and Thuridilla lineolate were able to inhibit the growth of the Staphylococcus aureus and Escherichia coli test. Keywords: nudibranch, Phyllidiella pustulosa, Thuridilla lineolate, bacteria, antibacterialAbstrakNudibranch adalah avetebrata laut dalam kelas Gastropoda yang mampu melakukan kamuflase dan mengembangkan sistem pertahanan diri. Bakteri laut mengandung senyawa kimia yang berpotensi sebagai bahan obat-obatan yang dihasilkan melalui metabolit sekunder. Tujuan dari penelitian ini yaitu untuk mengisolasi bakteri yang bersimbion dengan nudibranch Phylidiella pustulosa dan Thuridilla lineolate, kemudian mengamati aktivitas antibakteri dari isolat bakteri tersebut terhadap Escherichia coli danStaphylococcus aureus. Isolasi dan kultur bakteri yang bersimbion dibuat pada media B1 (padat). Skrining aktivitas antibakteri menunjukkan isolat bakteri yang bersimbion dengan nudibranch Phylidiella pustulosa dan Thuridilla lineolate mampu menghambat pertumbuhan organisme uji Staphylococcus aureus dan Escherichia coli. Kata kunci: nudibranch, Phyllidiella pustulosa, Thuridilla lineolate, bakteri, antibakteri
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