Ag掺杂对APMOCVD在Ag/Si衬底上生长的ZnO纳米结构细胞毒性的影响

Q4 Biochemistry, Genetics and Molecular Biology
K. Naumenko, A. Ievtushenko, V. Karpyna, O. Bykov, L. Myroniuk
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Pure and Ag-doped ZnO nanostructures were grown on Ag/Si substrates by atmospheric pressure metalorganic chemical vapor deposition method using a mixture of zinc acetylacetonate and silver acetylacetonate powders as a precursor. Argentum thin films were deposited on Si substrates by a thermal evaporation method. MTT-assay was used for the analysis of MDBK and MDCK cell viability in the definition of zinc oxide nanostructure cytotoxicity. Results. Ag-doped zinc oxide nanostructures were grown and characterized by X-ray diff raction, scanning electron microscopy, and energy dispersive X-ray spectroscopy. It was found that Si substrate and pure zinc oxide do not inhibit the cell viability of both epithelial cultures whereas Ag-doped ZnO nanostructures inhibit the cell viability because of all-time exposure in a sample without dilution. The cytotoxic effect was not observed at higher dilutions for Ag-doped zinc oxide nanostructures. Conclusions. 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引用次数: 1

摘要

寻找和开发新的纳米结构和纳米材料对纳米技术和现代微生物学的发展具有重要意义。由于银和氧化锌的独特性质,这些纳米颗粒是创建具有潜在抗病毒活性的纳米结构的最佳基础。这些研究中的一个重要问题是建立这些纳米颗粒及其复合材料的细胞毒性。的目标。研究底物温度和ZnO晶格中Ag浓度对氧化锌纳米结构的微观结构和细胞毒性的影响。方法。以乙酰丙酮锌和乙酰丙酮银混合粉末为前驱体,采用常压金属有机化学气相沉积法在Ag/Si衬底上生长出纯ZnO和掺银ZnO纳米结构。采用热蒸发法在硅衬底上沉积了银薄膜。在氧化锌纳米结构细胞毒性定义中,采用mtt法分析MDBK和MDCK细胞活力。结果。采用x射线衍射、扫描电镜和能量色散x射线能谱等方法对掺杂银氧化锌纳米结构进行了生长和表征。研究发现,硅衬底和纯氧化锌不会抑制两种上皮细胞培养物的细胞活力,而ag掺杂ZnO纳米结构由于长期暴露在未稀释的样品中而抑制细胞活力。在较高的稀释度下,对银掺杂的氧化锌纳米结构没有观察到细胞毒性作用。结论。研究ag掺杂对氧化锌纳米结构形貌和细胞毒性的影响,对于将氧化锌纳米结构应用于光电子和光催化领域具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Effect of Ag-Doping on the Cytotoxicity of ZnO Nanostructures Grown on Ag/Si Substrates by APMOCVD
The search and development of new nanostructures and nanomaterials are very important for the progress of nanotechnology and modern microbiology. Due to the unique properties of silver and zinc oxide, these nanoparticles are the optimal basis for creating nanostructures with potential antiviral activity. An important issue in these studies is the establishment of cytotoxicity of these nanoparticles and their composites. Aim. To define the influence of substrate temperature and Ag concentration in ZnO lattice on the microstructure and cytotoxicity of zinc oxide nanostructures. Methods. Pure and Ag-doped ZnO nanostructures were grown on Ag/Si substrates by atmospheric pressure metalorganic chemical vapor deposition method using a mixture of zinc acetylacetonate and silver acetylacetonate powders as a precursor. Argentum thin films were deposited on Si substrates by a thermal evaporation method. MTT-assay was used for the analysis of MDBK and MDCK cell viability in the definition of zinc oxide nanostructure cytotoxicity. Results. Ag-doped zinc oxide nanostructures were grown and characterized by X-ray diff raction, scanning electron microscopy, and energy dispersive X-ray spectroscopy. It was found that Si substrate and pure zinc oxide do not inhibit the cell viability of both epithelial cultures whereas Ag-doped ZnO nanostructures inhibit the cell viability because of all-time exposure in a sample without dilution. The cytotoxic effect was not observed at higher dilutions for Ag-doped zinc oxide nanostructures. Conclusions. The investigation of the effect of Ag-doping on the morphology and cytotoxicity of zinc oxide nanostructures is very important for implementing zinc oxide nanostructures into the current optoelectronics and photocatalysis.
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来源期刊
Mikrobiolohichnyi zhurnal
Mikrobiolohichnyi zhurnal Medicine-Microbiology (medical)
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