Natthawat Sritongon, S. Boonlue, W. Mongkolthanaruk, S. Jogloy, N. Riddech
{"title":"用变性梯度凝胶电泳研究根际细菌接种剂和有机改进剂对菊芋组织和根际细菌群落的影响","authors":"Natthawat Sritongon, S. Boonlue, W. Mongkolthanaruk, S. Jogloy, N. Riddech","doi":"10.12982/cmjs.2023.051","DOIUrl":null,"url":null,"abstract":"The application of organic amendments can improve soil quality and influence bacterial communities, contributing to enhanced soil health in agricultural systems. This study investigated the effect of using rain tree leaf, corn husk, and peat moss as soil amendments, supplemented with plant growth promoting rhizobacteria on communities of bacteria in the tissue and rhizosphere of Jerusalem artichoke. The plants were observed for 120 days after transplanting. Polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) technique was used to investigate the impact of both organic amendments and bacterial consortium with comparative profiles. Comparison DGGE profile by clustering analysis did not clearly separate each organic amendment and bacterial inoculation, but principal component analysis (PCA) revealed that plant age had an influence on tissue and rhizosphere bacterial communities. The results indicated that soil amendments and bacterial inoculation had no effect on bacterial community shifts. Interestingly, the genera of bacteria able to most colonize in the tissue and rhizosphere were the uncultured bacteria. Thus, we provide promising data for boosting Jerusalem artichoke growth and soil quality with potential organic amendments to improve soil with sustainable strategies.","PeriodicalId":9884,"journal":{"name":"Chiang Mai Journal of Science","volume":"18 1","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Impact of Rhizobacterial Inoculants and Organic Amendment on Bacterial Communities of Jerusalem artichoke Tissue and Rhizosphere by using Denaturing Gradient Gel Electrophoresis\",\"authors\":\"Natthawat Sritongon, S. Boonlue, W. Mongkolthanaruk, S. Jogloy, N. Riddech\",\"doi\":\"10.12982/cmjs.2023.051\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The application of organic amendments can improve soil quality and influence bacterial communities, contributing to enhanced soil health in agricultural systems. This study investigated the effect of using rain tree leaf, corn husk, and peat moss as soil amendments, supplemented with plant growth promoting rhizobacteria on communities of bacteria in the tissue and rhizosphere of Jerusalem artichoke. The plants were observed for 120 days after transplanting. Polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) technique was used to investigate the impact of both organic amendments and bacterial consortium with comparative profiles. Comparison DGGE profile by clustering analysis did not clearly separate each organic amendment and bacterial inoculation, but principal component analysis (PCA) revealed that plant age had an influence on tissue and rhizosphere bacterial communities. The results indicated that soil amendments and bacterial inoculation had no effect on bacterial community shifts. Interestingly, the genera of bacteria able to most colonize in the tissue and rhizosphere were the uncultured bacteria. Thus, we provide promising data for boosting Jerusalem artichoke growth and soil quality with potential organic amendments to improve soil with sustainable strategies.\",\"PeriodicalId\":9884,\"journal\":{\"name\":\"Chiang Mai Journal of Science\",\"volume\":\"18 1\",\"pages\":\"\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2023-09-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chiang Mai Journal of Science\",\"FirstCategoryId\":\"103\",\"ListUrlMain\":\"https://doi.org/10.12982/cmjs.2023.051\",\"RegionNum\":4,\"RegionCategory\":\"综合性期刊\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chiang Mai Journal of Science","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.12982/cmjs.2023.051","RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
Impact of Rhizobacterial Inoculants and Organic Amendment on Bacterial Communities of Jerusalem artichoke Tissue and Rhizosphere by using Denaturing Gradient Gel Electrophoresis
The application of organic amendments can improve soil quality and influence bacterial communities, contributing to enhanced soil health in agricultural systems. This study investigated the effect of using rain tree leaf, corn husk, and peat moss as soil amendments, supplemented with plant growth promoting rhizobacteria on communities of bacteria in the tissue and rhizosphere of Jerusalem artichoke. The plants were observed for 120 days after transplanting. Polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) technique was used to investigate the impact of both organic amendments and bacterial consortium with comparative profiles. Comparison DGGE profile by clustering analysis did not clearly separate each organic amendment and bacterial inoculation, but principal component analysis (PCA) revealed that plant age had an influence on tissue and rhizosphere bacterial communities. The results indicated that soil amendments and bacterial inoculation had no effect on bacterial community shifts. Interestingly, the genera of bacteria able to most colonize in the tissue and rhizosphere were the uncultured bacteria. Thus, we provide promising data for boosting Jerusalem artichoke growth and soil quality with potential organic amendments to improve soil with sustainable strategies.
期刊介绍:
The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.