{"title":"虎杖通过下调 Akt/mTOR 信号通路对胶质母细胞瘤癌细胞的抗增殖作用","authors":"Sevgi Irtegun Kandemir, Polat Ipek","doi":"10.4103/jcrt.jcrt_1886_21","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Glioblastoma multiforme (GBM) is the most aggressive brain tumor that is common among adults. This aggression is due to increased invasion, migration, proliferation, angiogenesis, and decreased apoptosis. Plant-based compounds have a high potential to be used as an anticancer agent due to their various mechanisms and less undesirable side effects. Potentilla fulgens is a medicinal plant, and methanolic root extract of P. fulgens (PRE) has anti-inflammatory and anticancer properties.</p><p><strong>Objective: </strong>In this study, we aimed to investigate antiproliferative effect of PRE on U118 and T98G glioblastoma cancer cells and to reveal which molecular signaling pathways regulate this mechanism of action.</p><p><strong>Materials and methods: </strong>The effect of PRE on cell viability of GBM cells was investigated by MTT assay. Involvement of PRE with cell growth and survival signaling pathways, phosphatidylinositol 3-kinase (PI3K)/Akt/mTOR and c-Src/signal transducer and activator of transcription 3 (STAT3), was examined using Western Blot.</p><p><strong>Results: </strong>PRE reduced cell viability of GBM and human dermal fibroblast (HDF) cells in a dose-and time-independent manner. PI3K expression/phosphorylation level remained unchanged in both GBM and HDF cells after PRE treatment, but Akt/mTOR signaling pathway was downregulated in PRE-treated cells. PRE treatment did not affect c-Src expression/phosphorylation level in GBM cells; however, expression of c-Src was suppressed in HDF cells. Similar results were observed for STAT3 expression and phosphorylation status.</p><p><strong>Conclusion: </strong>PRE has the ability to suppress cell viability in GBM cells, by targeting the Akt/mTOR signaling pathway.</p>","PeriodicalId":15208,"journal":{"name":"Journal of cancer research and therapeutics","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antiproliferative effect of Potentilla fulgens on glioblastoma cancer cells through downregulation of Akt/mTOR signaling pathway.\",\"authors\":\"Sevgi Irtegun Kandemir, Polat Ipek\",\"doi\":\"10.4103/jcrt.jcrt_1886_21\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Glioblastoma multiforme (GBM) is the most aggressive brain tumor that is common among adults. This aggression is due to increased invasion, migration, proliferation, angiogenesis, and decreased apoptosis. Plant-based compounds have a high potential to be used as an anticancer agent due to their various mechanisms and less undesirable side effects. Potentilla fulgens is a medicinal plant, and methanolic root extract of P. fulgens (PRE) has anti-inflammatory and anticancer properties.</p><p><strong>Objective: </strong>In this study, we aimed to investigate antiproliferative effect of PRE on U118 and T98G glioblastoma cancer cells and to reveal which molecular signaling pathways regulate this mechanism of action.</p><p><strong>Materials and methods: </strong>The effect of PRE on cell viability of GBM cells was investigated by MTT assay. Involvement of PRE with cell growth and survival signaling pathways, phosphatidylinositol 3-kinase (PI3K)/Akt/mTOR and c-Src/signal transducer and activator of transcription 3 (STAT3), was examined using Western Blot.</p><p><strong>Results: </strong>PRE reduced cell viability of GBM and human dermal fibroblast (HDF) cells in a dose-and time-independent manner. PI3K expression/phosphorylation level remained unchanged in both GBM and HDF cells after PRE treatment, but Akt/mTOR signaling pathway was downregulated in PRE-treated cells. PRE treatment did not affect c-Src expression/phosphorylation level in GBM cells; however, expression of c-Src was suppressed in HDF cells. Similar results were observed for STAT3 expression and phosphorylation status.</p><p><strong>Conclusion: </strong>PRE has the ability to suppress cell viability in GBM cells, by targeting the Akt/mTOR signaling pathway.</p>\",\"PeriodicalId\":15208,\"journal\":{\"name\":\"Journal of cancer research and therapeutics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2023-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of cancer research and therapeutics\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.4103/jcrt.jcrt_1886_21\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/7/15 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"ONCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of cancer research and therapeutics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4103/jcrt.jcrt_1886_21","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/7/15 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"ONCOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
背景:多形性胶质母细胞瘤(GBM多形性胶质母细胞瘤(GBM)是成人中常见的侵袭性最强的脑肿瘤。这种侵袭性是由于侵袭、迁移、增殖、血管生成增加以及凋亡减少造成的。植物性化合物具有多种机制,且不良副作用较小,因此很有可能被用作抗癌剂。黄花菜是一种药用植物,其甲醇根提取物(PRE)具有抗炎和抗癌特性:本研究旨在探讨 PRE 对 U118 和 T98G 胶质母细胞瘤癌细胞的抗增殖作用,并揭示调控这一作用机制的分子信号通路:MTT试验研究了PRE对GBM细胞活力的影响。利用 Western Blot 检测了 PRE 与细胞生长和存活信号通路(磷脂酰肌醇 3- 激酶(PI3K)/Akt/mTOR 和 c-Src/signal transducer and activator of transcription 3 (STAT3))的关系:结果:PRE以剂量和时间无关的方式降低了GBM和人真皮成纤维细胞(HDF)的细胞活力。PRE处理后,GBM和HDF细胞中的PI3K表达/磷酸化水平保持不变,但PRE处理的细胞中Akt/mTOR信号通路下调。PRE 处理不影响 GBM 细胞中 c-Src 的表达/磷酸化水平;但 HDF 细胞中 c-Src 的表达受到抑制。STAT3 的表达和磷酸化状态也观察到了类似的结果:结论:PRE 能够通过靶向 Akt/mTOR 信号通路抑制 GBM 细胞的活力。
Antiproliferative effect of Potentilla fulgens on glioblastoma cancer cells through downregulation of Akt/mTOR signaling pathway.
Background: Glioblastoma multiforme (GBM) is the most aggressive brain tumor that is common among adults. This aggression is due to increased invasion, migration, proliferation, angiogenesis, and decreased apoptosis. Plant-based compounds have a high potential to be used as an anticancer agent due to their various mechanisms and less undesirable side effects. Potentilla fulgens is a medicinal plant, and methanolic root extract of P. fulgens (PRE) has anti-inflammatory and anticancer properties.
Objective: In this study, we aimed to investigate antiproliferative effect of PRE on U118 and T98G glioblastoma cancer cells and to reveal which molecular signaling pathways regulate this mechanism of action.
Materials and methods: The effect of PRE on cell viability of GBM cells was investigated by MTT assay. Involvement of PRE with cell growth and survival signaling pathways, phosphatidylinositol 3-kinase (PI3K)/Akt/mTOR and c-Src/signal transducer and activator of transcription 3 (STAT3), was examined using Western Blot.
Results: PRE reduced cell viability of GBM and human dermal fibroblast (HDF) cells in a dose-and time-independent manner. PI3K expression/phosphorylation level remained unchanged in both GBM and HDF cells after PRE treatment, but Akt/mTOR signaling pathway was downregulated in PRE-treated cells. PRE treatment did not affect c-Src expression/phosphorylation level in GBM cells; however, expression of c-Src was suppressed in HDF cells. Similar results were observed for STAT3 expression and phosphorylation status.
Conclusion: PRE has the ability to suppress cell viability in GBM cells, by targeting the Akt/mTOR signaling pathway.
期刊介绍:
The journal will cover technical and clinical studies related to health, ethical and social issues in field of Medical oncology, radiation oncology, medical imaging, radiation protection, non-ionising radiation, radiobiology. Articles with clinical interest and implications will be given preference.