钙、BAP和腐胺对巨尾桉杂种幼体外植体胚诱导的影响

L. C. Moura, Aloisio Xavier, A. C. F. Cruz, D. S. Batista, R. Gallo, N. A. Miranda, W. Otoni
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引用次数: 4

摘要

考虑到桉树克隆技术的不断改进和对该物种生产植株的新技术的探索,体细胞胚胎发生已经吸引了使用先进遗传育种和克隆计划的研究小组和林业公司的兴趣。本研究的目的是验证钙的浓度和来源、细胞分裂素BAP和多胺腐胺的浓度和作用时间对尾叶巨桉幼体外植体胚诱导和发育的影响。子叶外植体接种于以氯化钙(MS培养基)或硝酸钙(JADS培养基)为钙源的培养基中。在MS培养基中,硝酸钙浓度分别为4.40 gL-1(对照-Ca)、6.60 gL-1(比对照-Ca 50增加50%)和8.80 gL-1(比对照-Ca 100增加100%);JADS培养基中氯化钙含量分别为:11.81 gL-1 (Ca)、17.72 gL-1 (Ca50)和23.62 gL-1 (Ca100)。将子叶外植体接种到含有20.71 μM picloram作为生长调节剂的初级诱导培养基(PIM)中。初生诱导10、20和30 d时,将外植体转移到含有20.71 μM picloram和11.10 μM BAP或28.36 μM腐胺的二次诱导培养基(SIM)上。与含有氯化钙的培养基相比,含有硝酸钙的培养基具有更高的钙形成率。培养基中钙浓度的增加并没有提高体细胞前胚胎的诱导率。而在培养基中添加28.36 μM的腐胺,诱导体细胞胚胎发生的比例更高。培养基中添加BAP和腐胺后,每个外植体形成的体细胞前胚数高于只添加picloram的培养基。为了获得更多的巨尾桉体细胞前胚,应使用含有28.36 μM腐殖酸的JADS培养基。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of calcium, BAP and putrescine on somatic embryo induction in juvenile explants of Eucalyptus grandis × E. urophylla hybrids
Considering the constant improvement of Eucalyptus cloning and the search for new technologies to produce plantlets of this species, somatic embryogenesis has attracted interest from research groups and forestry companies that use advanced genetic breeding and cloning programs. The objective of the present study was to verify the effect of concentrations and sources of calcium, concentrations and effect time of cytokinin BAP and polyamine putrescine on the induction and development of somatic embryos in juvenile explants of Eucalyptus grandis x E. urophylla. Cotyledonary explants were inoculated into culture medium containing calcium chloride (MS medium) or calcium nitrate (JADS medium) as source of calcium. Different concentrations of calcium were also used, for MS medium containing: 4.40 gL-1 (control-Ca), 6.60 gL-1 (50% increase over control - Ca 50) and 8.80 gL-1 (increase of 100% over control – Ca 100) of calcium nitrate; and for JADS medium containing: 11.81 gL-1 (Ca), 17.72 gL-1 (Ca50) and 23.62 gL-1 (Ca100) of calcium chloride. Cotyledon explants were inoculated into the primary induction medium (PIM) containing 20.71 μM picloram as growth regulator. At 10, 20 and 30 days of primary induction, the explants were transferred to the secondary induction medium (SIM) containing 20.71 μM picloram and 11.10 μM BAP or 28.36 μM putrescine. The culture medium containing calcium nitrate provided higher callogenesis when compared to the medium containing calcium chloride. The increase in calcium concentration in the media did not provide higher percentage of induction of somatic pro-embryos. However, the addition of 28.36 μM putrescine to the culture medium provided a higher percentage of induction of somatic embryogenesis. The number of somatic pro-embryos formed per explant was higher when BAP and putrescine were added to the culture medium when compared to medium containing only picloram. To obtain a greater number of somatic pro-embryos of Eucalyptus grandis × E. urophylla, the JADS culture medium containing 28.36 μM putrecine should be used.
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