Biosynthesis of Silver Nanoparticles Using Mentha longifolia (L.) Hudson Leaf Extract and Study its Antibacterial Activity

In the present study, a simple, fast and eco-friendly biosynthesis of silver nanoparticles (AgNPs) using   Mentha langifolia leaf extract as the reducing agent was investigated. Nanoparticles forming were indicated by the color changes in solution and were confirmed by UV-Vis spectrum, FT-IR analysis and Scanning Electron Microscopic (SEM) micrograph. The average particle size of produced AgNPs was determined by dynamic light scattering (DLS) technique. Further, the antibacterial activity of synthesized AgNPs was evaluated against Streptococcus pneumonia and staphylococcus epidermidis as Gram-positive bacteria and Salmonella enterica and enterobacter aerogenes as Gram-negative bacteria. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of biosynthesized silver nanoparticles were determined. UV-Visible spectrophotometer showed absorbance peak in 340 nm. DLS analysis indicated that the average size of AgNPs is 21.1nm. The results of SEM showed that synthesized AgNPs are spherical in shape. The antibacterial activities of the silver nanoparticles were studied against subject bacteria. The present report explores a rapid, simple and economical route, without any hazardous chemicals as reducing or stabilizing agents to synthesis AgNPs and describes the antimicrobial activities of synthetized silver nanoparticles.