固定化酿酒酵母在预处理甘蔗渣上生产生物乙醇的研究[j]

S. Anita, W. Mangunwardoyo, Yopi Yopi
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引用次数: 1

摘要

以预处理过的甘蔗渣为载体,固定化酿酒酵母。采用蒸汽、加压蒸汽和两种方法相结合的预处理方法。本研究旨在探讨预处理蔗渣对细胞吸附和生物乙醇生产的影响。固定过程以2.5 g载体/50 mL细胞悬液的比例进行。用1% (w/v)的全细胞生物催化剂作为接种物进行生物乙醇发酵。经预处理的甘蔗渣蒸汽处理30 min,获得了最佳的固定化细胞载体。这些处理改善了载体的物理性能,提高了细胞保留率,达到10.05 mg/g。蒸汽预处理后使用全细胞生物催化剂,乙醇收率也比对照提高了1.5倍。关键词:生物乙醇;固定;预处理;蒸汽处理;甘蔗蔗渣
本文章由计算机程序翻译,如有差异,请以英文原文为准。
IMPROVEMENT OF BIOETHANOL PRODUCTION BY USING Saccharomyces cerevisiae [Meyen ex E.C. Hansen] IMMOBILIZED ON PRETREATED SUGARCANE BAGASSE
Pretreated of sugarcane bagasse was used as a carrier for immobilization of Saccharomyces cerevisiae. Pretreatments were carried out by steaming, pressurized steam, and combination both of procedure.  The objectives of this research was to investigate the effect of pretreatment on sugarcane bagasse to cells adsorption and bioethanol production.  Immobilization process was conducted in a ratio of 2.5 g carrier/50 mL cell suspension.  Whole cell biocatalyst as much as 1% (w/v) was used as inoculum for bioethanol fermentation.  The best pretreated sugarcane bagasse for carrier of immobilized cells was obtained using steam treatment for 30 minutes.  Those treatment improved the physical properties of carrier and increased the cell retention up to 10.05 mg/g.  The use of whole cell biocatalyst after steaming pretreatment also enhanced ethanol yield 1.5 times higher than control. Keywords: bioethanol; immobilization; pretreatment; steam treatment; sugarcane bagasse
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