表达蝎子毒素LqhIT2的重组杆状病毒的杀虫效果

N. Imai, S. Ali, N. El-Singaby, M. Iwanaga, S. Matsumoto, K. Iwabuchi, S. Maeda
{"title":"表达蝎子毒素LqhIT2的重组杆状病毒的杀虫效果","authors":"N. Imai, S. Ali, N. El-Singaby, M. Iwanaga, S. Matsumoto, K. Iwabuchi, S. Maeda","doi":"10.11416/KONTYUSHIGEN1930.69.197","DOIUrl":null,"url":null,"abstract":"A system of the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV) and the silkworm larvae, B. mori, was applied to analyze the potential use of the insect-specific toxin LghIT2 for improving the efficiency of recombinant baculoviruses for pest control. The LghIT2 gene, along with the signal sequence for secretion, was synthesized and transferred into the BmNPV genome to generate the recombinant virus BmLqhIT2. Larvae infected with BmLqhIT2 showed LqhIT2-specific symptoms at 48 h post injection (p. i.), stopped feeding at 60 h p. i., and became motionless by 84 h p. i. Particularly, the larvae infected with BmLghIT2 stopped moving 24 h earlier than those infected with BmAaIT, which expressed another insect-specific scorpion toxin AaIT. An N-terminal sequence and mass spectrometric analyses showed that BmLqhIT2 produced mature LghIT2. The present results suggest that LqhIT2 is more efficient than AaIT for improving the Insecticidal activity of baculoviruses.","PeriodicalId":22665,"journal":{"name":"The journal of sericultural science of Japan","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2000-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"8","resultStr":"{\"title\":\"Insecticidal effects of a recombinant baculovirus expressing scorpion toxin LqhIT2\",\"authors\":\"N. Imai, S. Ali, N. El-Singaby, M. Iwanaga, S. Matsumoto, K. Iwabuchi, S. Maeda\",\"doi\":\"10.11416/KONTYUSHIGEN1930.69.197\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A system of the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV) and the silkworm larvae, B. mori, was applied to analyze the potential use of the insect-specific toxin LghIT2 for improving the efficiency of recombinant baculoviruses for pest control. The LghIT2 gene, along with the signal sequence for secretion, was synthesized and transferred into the BmNPV genome to generate the recombinant virus BmLqhIT2. Larvae infected with BmLqhIT2 showed LqhIT2-specific symptoms at 48 h post injection (p. i.), stopped feeding at 60 h p. i., and became motionless by 84 h p. i. Particularly, the larvae infected with BmLghIT2 stopped moving 24 h earlier than those infected with BmAaIT, which expressed another insect-specific scorpion toxin AaIT. An N-terminal sequence and mass spectrometric analyses showed that BmLqhIT2 produced mature LghIT2. The present results suggest that LqhIT2 is more efficient than AaIT for improving the Insecticidal activity of baculoviruses.\",\"PeriodicalId\":22665,\"journal\":{\"name\":\"The journal of sericultural science of Japan\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2000-06-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The journal of sericultural science of Japan\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.11416/KONTYUSHIGEN1930.69.197\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The journal of sericultural science of Japan","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.11416/KONTYUSHIGEN1930.69.197","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 8

摘要

采用家蚕杆状病毒核多角体病毒(BmNPV)和家蚕幼虫组成的系统,分析了昆虫特异性毒素LghIT2在提高重组杆状病毒防治害虫效率方面的潜在应用价值。合成LghIT2基因和分泌信号序列,并将其转移到BmNPV基因组中,生成重组病毒BmLqhIT2。感染BmLqhIT2的幼虫在注射后48 h (p. i.)出现lqhit2特异性症状,60 h停止进食,84 h静止不动,特别是感染BmLqhIT2的幼虫比感染表达另一种昆虫特异性蝎子毒素ait的BmAaIT早24 h停止运动。n端序列和质谱分析表明BmLqhIT2产生成熟的LghIT2。结果表明,LqhIT2比ait更能提高杆状病毒的杀虫活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Insecticidal effects of a recombinant baculovirus expressing scorpion toxin LqhIT2
A system of the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV) and the silkworm larvae, B. mori, was applied to analyze the potential use of the insect-specific toxin LghIT2 for improving the efficiency of recombinant baculoviruses for pest control. The LghIT2 gene, along with the signal sequence for secretion, was synthesized and transferred into the BmNPV genome to generate the recombinant virus BmLqhIT2. Larvae infected with BmLqhIT2 showed LqhIT2-specific symptoms at 48 h post injection (p. i.), stopped feeding at 60 h p. i., and became motionless by 84 h p. i. Particularly, the larvae infected with BmLghIT2 stopped moving 24 h earlier than those infected with BmAaIT, which expressed another insect-specific scorpion toxin AaIT. An N-terminal sequence and mass spectrometric analyses showed that BmLqhIT2 produced mature LghIT2. The present results suggest that LqhIT2 is more efficient than AaIT for improving the Insecticidal activity of baculoviruses.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信