{"title":"微流式细胞仪的CAD研究及其在细菌检测中的应用","authors":"S.S. Lee, S. Izuo, K. Inatomi","doi":"10.1109/ICSENS.2004.1426163","DOIUrl":null,"url":null,"abstract":"We have designed and fabricated a micro flow cytometer for application to bacteria detection. In this paper, we present a CAD study which has been done to obtain optimal working conditions of the device. In the simulation, we have investigated flow rate ratios of sample and sheath flow to confirm hydrodynamic focusing at a desired position and proper sample stream width at the detection position. As a result, we could obtain adequate flow rate ratios of sample and sheath flow, which is in the vicinity of 1:5 for our device. A bacteria enumeration experiment has also been carried out, based on the simulation results, and we have achieved hydrodynamic focusing at the expected position and proper sample flow rate.","PeriodicalId":20476,"journal":{"name":"Proceedings of IEEE Sensors, 2004.","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"A CAD study on micro flow cytometer and its application to bacteria detection\",\"authors\":\"S.S. Lee, S. Izuo, K. Inatomi\",\"doi\":\"10.1109/ICSENS.2004.1426163\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We have designed and fabricated a micro flow cytometer for application to bacteria detection. In this paper, we present a CAD study which has been done to obtain optimal working conditions of the device. In the simulation, we have investigated flow rate ratios of sample and sheath flow to confirm hydrodynamic focusing at a desired position and proper sample stream width at the detection position. As a result, we could obtain adequate flow rate ratios of sample and sheath flow, which is in the vicinity of 1:5 for our device. A bacteria enumeration experiment has also been carried out, based on the simulation results, and we have achieved hydrodynamic focusing at the expected position and proper sample flow rate.\",\"PeriodicalId\":20476,\"journal\":{\"name\":\"Proceedings of IEEE Sensors, 2004.\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2004-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Proceedings of IEEE Sensors, 2004.\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/ICSENS.2004.1426163\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of IEEE Sensors, 2004.","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/ICSENS.2004.1426163","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A CAD study on micro flow cytometer and its application to bacteria detection
We have designed and fabricated a micro flow cytometer for application to bacteria detection. In this paper, we present a CAD study which has been done to obtain optimal working conditions of the device. In the simulation, we have investigated flow rate ratios of sample and sheath flow to confirm hydrodynamic focusing at a desired position and proper sample stream width at the detection position. As a result, we could obtain adequate flow rate ratios of sample and sheath flow, which is in the vicinity of 1:5 for our device. A bacteria enumeration experiment has also been carried out, based on the simulation results, and we have achieved hydrodynamic focusing at the expected position and proper sample flow rate.
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