木薯褐条病症状变异及病毒侵染与症状表达关系的研究

G. Rwegasira
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摘要

木薯褐条病(CBSD)的诊断长期以来都是基于患病植株的叶和根症状表达。然而,品种和季节之间症状表现模式的变化意味着基于症状的诊断是不可靠的。目前的研究确定了与CBSD相关的三种主要类型的叶子症状(绿斑、绿斑、静脉绿斑)和根症状(褐黑色坏死、白垩坏死、坏死斑点)。新描述的CBSD症状包括;斑状叶褪绿,叶柄基部的褐色坏死的内部组织和白垩色的根坏死。通过RT-PCR建立了症状表达与CBSV存在的关系。确定了最适合用RT-PCR检测CBSV的植物部位。叶片CBSD症状可能是CBSV感染的不可靠指示。此外,CBSV在感染植株内的分布可能不均匀。多达67%的检测样本来自有症状和目测被CBSV感染的植物。大约22%的人没有CBSV,尽管有明显的CBSD症状,只有7%的人感染了CBSV,但没有症状。CBSV在花、果实、顶芽、嫩叶、新开叶、最年轻有症状叶、茎的嫩顶绿色部分和未坏死的贮藏根组织中检测到。本研究认为,基于症状的诊断应考虑叶片和根系的检查,并始终通过RT-PCR的分子检测来证实。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Studies on Variability of Cassava Brown Streak Disease Symptoms and the Relationship between Virus Infection and Symptom Expression on Cassava Crop
Diagnosis of cassava brown streak disease (CBSD) has for long been based on foliage and root symptoms expression by infected plants. Variability in patterns of symptom expression between varieties and seasons however, has meant that symptom-based diagnosis is unreliable. The current study identified three major types of foliage symptoms (chlorotic blotches, chlorotic spots, vein chlorosis) and root (brownish black necrosis, chalky necrosis, necrotic specks) symptoms associated with CBSD. The newly described CBSD symptoms includes; spotty foliage chlorosis, brown necrotic internal tissue at the base of the leaf petiole and chalky root necrosis. The relationship between symptom expression and the presence of Cassava brown streak virus (CBSV) through RT-PCR was also established. Plant parts most suitable for detection of CBSV by RT-PCR were identified. Foliage CBSD symptoms could be unreliable indication of CBSV infection. Moreover, CBSV may not be uniformly distributed within the infected plants. Up to 67% of tested samples were from plants that were both symptomatic and visually seen to be infected by CBSV. About 22% were free from CBSV despite apparently exhibiting CBSD symptoms and only 7% were CBSV-infected but symptomless. CBSV was detected in flowers, fruits, apical buds, young tender leaves, newly-opened leaves, youngest symptomatic leaves, and the tender top green portion of the stem and non-necrotic storage root tissues. The study concluded that symptom based diagnosis for CBSD should consider examination of both foliage and roots and always be confirmed with molecular detection by RT-PCR.
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