Isolation and screening of pyocyanin producing Pseudomonas spp. from soil

Pseudomonas spp. produces large quantities of water soluble blue green phenazine pigment pyocyanin. The blue green chloroform soluble phenazine pigment extracted from Pseudomonas spp has antimicrobial activity. In present study a total of nine bacterial isolated screened from the different environment sample. The isolates obtained were gram negative, non-spore forming and exhibited fluorescence under UV light. The isolate showing blue green pigment on king’s B agar medium at a temperature of 35oC after 48-72 hr. of incubation and considered as having capabilities to produce pyocyanin.