{"title":"兼性厌氧菌低氧化还原电位细胞色素c552与氢化酶的相互关系","authors":"J.A. Cole, J.W.T. Wimpenny","doi":"10.1016/0926-6593(66)90002-6","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Cytochrome <span><math><mtext>c</mtext><msub><mi></mi><mn>552</mn></msub></math></span> and hydrogenase (EC 1.12.1.1) activities have been compared in <em>Escherichia coli</em> and <em>Escherichia aurescens</em> cells grown aerobically and anaerobically in both complex and defined media.</p></span></li><li><span>2.</span><span><p>2. Sodium formate added to the growth media stimulates synthesis of increased amounts of cytochrome <span><math><mtext>c</mtext><msub><mi></mi><mn>552</mn></msub></math></span>.</p></span></li><li><span>3.</span><span><p>3. Extensive washing in 0.1 M phosphate buffer (pH 7.4), extensive sonication, and washing with 0.2% w/v sodium deoxycholate all solubilise cytochrome <span><math><mtext>c</mtext></math></span> from hydrogenase-active membranes.</p></span></li><li><span>4.</span><span><p>4. Residues from the above procedures still show hydrogenase activity.</p></span></li><li><span>5.</span><span><p>5. Cells grown anaerobically with nitrate s terminal electron acceptor contain greatly enhanced amounts of cytochrome: these cells do not have hydrogenase, hydrogenlyase or soluble viologen-linked formic dehydrogenase activities.</p></span></li></ul><p>The relationship of cytochrome <span><math><mtext>c</mtext><msub><mi></mi><mn>552</mn></msub></math></span> to hydrogenase is discussed.</p></div>","PeriodicalId":100160,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation","volume":"128 3","pages":"Pages 419-425"},"PeriodicalIF":0.0000,"publicationDate":"1966-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6593(66)90002-6","citationCount":"41","resultStr":"{\"title\":\"The inter-relationships of low redox potential cytochrome c552 and hydrogenase in facultative anaerobes\",\"authors\":\"J.A. Cole, J.W.T. Wimpenny\",\"doi\":\"10.1016/0926-6593(66)90002-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. Cytochrome <span><math><mtext>c</mtext><msub><mi></mi><mn>552</mn></msub></math></span> and hydrogenase (EC 1.12.1.1) activities have been compared in <em>Escherichia coli</em> and <em>Escherichia aurescens</em> cells grown aerobically and anaerobically in both complex and defined media.</p></span></li><li><span>2.</span><span><p>2. Sodium formate added to the growth media stimulates synthesis of increased amounts of cytochrome <span><math><mtext>c</mtext><msub><mi></mi><mn>552</mn></msub></math></span>.</p></span></li><li><span>3.</span><span><p>3. Extensive washing in 0.1 M phosphate buffer (pH 7.4), extensive sonication, and washing with 0.2% w/v sodium deoxycholate all solubilise cytochrome <span><math><mtext>c</mtext></math></span> from hydrogenase-active membranes.</p></span></li><li><span>4.</span><span><p>4. Residues from the above procedures still show hydrogenase activity.</p></span></li><li><span>5.</span><span><p>5. Cells grown anaerobically with nitrate s terminal electron acceptor contain greatly enhanced amounts of cytochrome: these cells do not have hydrogenase, hydrogenlyase or soluble viologen-linked formic dehydrogenase activities.</p></span></li></ul><p>The relationship of cytochrome <span><math><mtext>c</mtext><msub><mi></mi><mn>552</mn></msub></math></span> to hydrogenase is discussed.</p></div>\",\"PeriodicalId\":100160,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation\",\"volume\":\"128 3\",\"pages\":\"Pages 419-425\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1966-12-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6593(66)90002-6\",\"citationCount\":\"41\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926659366900026\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926659366900026","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The inter-relationships of low redox potential cytochrome c552 and hydrogenase in facultative anaerobes
1.
1. Cytochrome and hydrogenase (EC 1.12.1.1) activities have been compared in Escherichia coli and Escherichia aurescens cells grown aerobically and anaerobically in both complex and defined media.
2.
2. Sodium formate added to the growth media stimulates synthesis of increased amounts of cytochrome .
3.
3. Extensive washing in 0.1 M phosphate buffer (pH 7.4), extensive sonication, and washing with 0.2% w/v sodium deoxycholate all solubilise cytochrome from hydrogenase-active membranes.
4.
4. Residues from the above procedures still show hydrogenase activity.
5.
5. Cells grown anaerobically with nitrate s terminal electron acceptor contain greatly enhanced amounts of cytochrome: these cells do not have hydrogenase, hydrogenlyase or soluble viologen-linked formic dehydrogenase activities.
The relationship of cytochrome to hydrogenase is discussed.
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