发酵米糠提取物的生物活性物质及其抗氧化性能

안희영, 최다정, 김보경, 이재홍, 조영수
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引用次数: 1

摘要

本研究表明,发酵米糠提取物含有天然抗氧化剂。以发酵米糠(FRB)提取物为原料,采用体外实验模型检测其生物活性物质(如多酚类化合物和类黄酮)含量、抗氧化性能(DPPH (α,α′-二苯基-β-苦酰肼)自由基清除能力、铁还原能力、铜还原能力、亚油酸过氧化能力和大鼠肝细胞微粒体)。黄酮类化合物和酚类化合物的产水浓度分别为19.92和11.56㎎/g。在体外氧化模型中采用DPPH自由基清除能力(自由基清除能力69.8%)、铁还原能力和铜还原能力(剂量依赖性作用)、铁2+ /抗坏血酸通过硫氰酸铁和硫代巴比妥酸(TBA)方法诱导的亚油酸过氧化(抑制活性81%)和大鼠肝微粒体膜的自氧化(脂质过氧化抑制活性38%)。FRB提取物的抗氧化活性明显强于FRS(发酵大米和大豆,阳性对照)提取物,且呈剂量依赖性。从这些结果来看,FRB提取物具有最有效的抗氧化特性,并且含有最多的抗氧化化合物,如酚类化合物和类黄酮。研究结果为进一步了解发酵米糠的抗氧化特性,开发功能性食品提供了基础数据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bioactive Materials and Antioxidant Properties of Fermented Rice-bran Extract
This study suggests that fermented rice bran extract contains natural antioxidants. The contents of bioactive materials (e.g., polyphenolic compounds and flavonoids), antioxidative properties (DPPH (α,α’-diphenyl-β-picrylhydrazyl) free radical scavenging activity, Fe reducing, Cu reducing power, peroxidation of linoleic acid and rat hepatocyte microsome) were tested by in vitro experimental models using fermented rice bran (FRB) extract. The concentrations of phenolic compound and flavonoid were 19.92 ㎎/g and 11.56 ㎎/g, respectively. In oxidation in vitro models using DPPH free radical scavenging activity, (free radical scavenging activity 69.8%) Fe reducing power and Cu reducing power (effect of dose-dependent manner), Fe 2+ /ascorbate induced linolenic acid peroxidation by ferric thiocyanate and thiobarbituric acid (TBA) methods (inhibition activity 81%), and autooxidation of rat hepatic microsomes membrane (lipid peroxidation inhibition activity 38%), antioxidative activities were stronger in FRB extract than FRS (Fermented Rice and Soybean, positive control) extract and, these effects were dose-dependent manner. From these results, FRB extract was shown to have the most potent antioxidative properties and contain the highest amounts of antioxidative compounds such as phenolic compounds and flavonoids. Overall, these results may provide the basic data to understand the antioxidative properties of fermented rice bran for development of functional foods.
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