印度东北部一种有发展前途的民族药用植物——野生头草真菌内生新分类群及其系统发育分析

Richa Sharma, S. Tangjang, A. C. Shukla
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引用次数: 1

摘要

本研究首次报道了民族药用植物头草真菌内生菌的多样性。(竹芋科)。这种植物广泛生长在印度** *的Papum Pare森林中,传统上被用于抗糖尿病、抗高血糖或镇痛作用。在调查过程中,采集植物的叶、茎、根等部分进行内生菌的分离,并根据其形态、培养和繁殖结构(透明状、椭球状、无菌状、柱头、分生孢子、周皮、子囊和子囊孢子)对内生菌进行鉴定。利用5.8S和28S rDNA内转录间隔序列1和4对分离种进行系统发育分析。从叶片中分离到的真菌内生菌最多(39%),其次是根(31%)和茎(30%)。共分离到35种真菌,其中34种属于子囊菌纲,1种属于接合菌纲。物种丰富度和定植频率均以叶片最高。结果表明,长拟盘多毛孢是最优势的内生菌种,其次是小精子双plodina hinnuleum、黄曲霉Aspergillus flavus、Diaporthe sp.、Nigrospora oryzae和Lasiodiplodia viticola。然而,忽略拟盘多毛孢、金缕索菌、小精子双plodina、incarnatum镰刀菌、Preussia sp.、Diaporthe sp.、Aureobasidium sp.是头藤中常见的内生真菌
本文章由计算机程序翻译,如有差异,请以英文原文为准。
New Taxon of Fungal Endophytes from Phrynium capitatum Willd: A Promising Ethnomedicinal Plant in Northeast India and its Systematic and Phylogenetic Analysis
The findings of the current study deal with the first time report on the diversity of fungal endophyte from the ethnomedicinal plant- Phrynium capitatum Willd., (Marantaceae). The plant is growing wildly in the forest of Papum Pare, Arunachal Pradesh, India, and traditionally been used for anti-diabetic, antihyperglycemic or analgesic effects. During the investigation, samples of the plant parts, viz., leaves, stem, and roots were collected for isolation of endophytic spp. The endophytic spp were identified based on the morphological, cultural, and reproductive structures (hyaline, ellipsoidal, aseptate, pycnidia, beta conidia, perithecia, asci, and ascospores). Further, the phylogenetic analysis of the isolated species was made, using the sequences of 5.8S and 28S rDNA internal transcribed spacer sequence 1 and 4. The largest number of fungal endophytes (39%) were isolated from the leaves, followed by the roots (31%) and stems (30%). Overall 35 fungal species have been isolated, out of which, thirty-four belongs to the class Ascomycetes, and one from the class zygomycetes. The highest species richness and frequency of colonization were recorded in the leaf. The observations show that Pestalotiopsis longiseta was the most dominant endophytic species followed by Diplodina microsperma, Nodulisporium hinnuleum, Aspergillus flavus , Diaporthe sp., Nigrospora oryzae and Lasiodiplodia viticola. However, Pestalotiopsis neglecta, Sordaria fimicola, Diplodina microsperma, Fusarium incarnatum, Preussia sp., Diaporthe sp., Aureobasidium sp.were common fungal endophytes isolated from P. capitatum
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