胍丁胺对顺铂诱导的听觉细胞系细胞凋亡的保护作用。

IF 2.1 3区 数学 Q1 MATHEMATICS, APPLIED
Euyhyun Park, Se Hee Lee, Hak Hyun Jung, Gi Jung Im
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引用次数: 0

摘要

本研究的目的是评价胍丁氨酸对顺铂诱导的细胞凋亡的保护作用,并证实胍丁氨酸的保护机制。方法采用不同浓度的胍丁氨酸和15 μM的顺铂共处理Corti 1细胞48 h。测定细胞活力和增殖能力。Annexin v -异硫氰酸荧光素/碘化丙啶染色分析细胞凋亡。流式细胞术检测细胞内活性氧水平。检测bcl2相关X蛋白表达及caspase-3酶活性,探讨凋亡诱导途径。结果正常情况下,10 mM的胍丁氨酸对小鼠的保护作用最大。然而,在顺铂存在的情况下,最大的保护作用是从8毫米的胍丁氨酸观察到的。因此,选择8 mM作为理想的胍丁氨酸浓度来分析对顺铂诱导的细胞毒性的保护作用。胍丁氨酸对15 μM顺铂作用48小时具有明显的保护作用,可减少坏死和晚期凋亡细胞的比例。胍丁氨酸没有显著降低顺铂诱导的活性氧的增加,但降低了bcl2相关X蛋白的表达和caspase-3的活性。结论胍丁氨酸对顺铂诱导的听觉细胞系细胞凋亡具有保护作用。这些作用是通过保护线粒体功能和抑制细胞凋亡介导的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protective Effect of Agmatine Against Cisplatin- Induced Cellular Apoptosis in an Auditory Cell Line.

Background: The aims of this study were to evaluate the protective effects of agmatine against cisplatin-induced cellular apoptosis in an audi- tory cell line and to prove the protective mechanism of agmatine.

Methods: The House Ear Institute-Organ of Corti 1 cells were co-treated with agmatine at different concentrations and 15 μM of cisplatin for 48 hours. Cell viability and proliferation were measured. Annexin V-fluorescein isothiocyanate /propidium iodide staining was performed to analyze apoptosis. The levels of intracellular reactive oxygen species were measured using flow cytometry. The expression of BCL2-associated X protein and the enzymatic activity of caspase-3 was measured to examine the pathway of apoptosis induction.

Results: In normal conditions, the maximal protective effect occurred with 10 mM of agmatine. However, in the presence of cisplatin, the maximal protective effect was observed from 8 mM of agmatine. Thus, 8 mM was chosen as the ideal agmatine concentration for the analysis of protective effects against cisplatin-induced cytotoxicity. Agmatine exerted a significant protective effect against 15 μM of cisplatin when applied for 48 hours and reduced the proportion of necrotic and late apoptotic cells. Agmatine did not significantly reduce the cisplatin-induced increase in reactive oxygen species but decreased the expression of BCL2-associated X protein and the activity of caspase-3.

Conclusion: Agmatine protected against cisplatin-induced cellular apoptosis in an auditory cell line. These effects were mediated by the pro- tection of mitochondrial function and inhibition of apoptosis.

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来源期刊
CiteScore
4.90
自引率
6.90%
发文量
798
审稿时长
6 months
期刊介绍: Mathematical Methods in the Applied Sciences publishes papers dealing with new mathematical methods for the consideration of linear and non-linear, direct and inverse problems for physical relevant processes over time- and space- varying media under certain initial, boundary, transition conditions etc. Papers dealing with biomathematical content, population dynamics and network problems are most welcome. Mathematical Methods in the Applied Sciences is an interdisciplinary journal: therefore, all manuscripts must be written to be accessible to a broad scientific but mathematically advanced audience. All papers must contain carefully written introduction and conclusion sections, which should include a clear exposition of the underlying scientific problem, a summary of the mathematical results and the tools used in deriving the results. Furthermore, the scientific importance of the manuscript and its conclusions should be made clear. Papers dealing with numerical processes or which contain only the application of well established methods will not be accepted. Because of the broad scope of the journal, authors should minimize the use of technical jargon from their subfield in order to increase the accessibility of their paper and appeal to a wider readership. If technical terms are necessary, authors should define them clearly so that the main ideas are understandable also to readers not working in the same subfield.
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