黄体酮在棘皮类Lytechinus variegatus Lamarck卵巢和睾丸中的代谢

Kristina M Wasson, Stephen A Watts
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引用次数: 25

摘要

在本研究中,我们检测了黄体酮(P4)在黄体酮卵巢和睾丸的匀浆和组织碎料中的代谢命运。P4在卵巢和睾丸中主要代谢为5α-孕酮-3,20-二酮(DHP)、3β-羟基-5α-孕酮-20-酮(3β,20-酮)、5α-孕酮-3β,20α-二醇(3β,20α-二醇)、5α-孕酮-3β,20β-二醇(3β,20β-二醇)和5α-孕酮-3α,20α-二醇(3α,20α-二醇)。卵巢和睾丸代谢P4的能力没有差异。卵巢和睾丸组织肉末合成的5α-孕烯-3β,20ξ-二醇的相对含量是匀浆制备的3.3倍左右。卵巢和睾丸中3β,20- 1和3α,20α-二醇的合成差异依赖于生殖状态。本研究证实了P4在L. variegatus的卵巢和睾丸中的转化途径,并提示P4在这些组织中迅速转化为5α-减少的代谢物。尽管P4代谢不是性别特异性的,但先前已经证明了对P4代谢物的性别特异性反应。我们假设卵巢和睾丸对P4的性别特异性反应可能与受体水平的调节过程有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Progesterone metabolism in the ovaries and testes of the echinoid Lytechinus variegatus Lamarck (Echinodermata)

In the present study we examined the metabolic fate of progesterone (P4) in homogenate and tissue minces of the ovaries and testes of Lytechinus variegatus. P4 was metabolized primarily into 5α-reduced metabolites including, 5α-pregnane-3,20-dione (DHP), 3β-hydroxy-5α-pregnan-20-one (3β,20-one), 5α-pregnane-3β,20α-diol (3β,20α-diol), 5α-pregnane-3β,20β-diol (3β,20β-diol), and 5α-pregnane-3α,20α-diol (3α,20α-diol) by both the ovaries and testes. The capacity to metabolize P4 did not differ between the ovaries and testes. However, the relative quantity of 5α-pregnane-3β,20ξ-diol synthesized from ovary and testis tissue minces was about 3.3-fold higher than from homogenate preparations. Differences in the synthesis of 3β,20-one and 3α,20α-diol in both ovary and testis minces were dependent on reproductive state. This study demonstrates the pathway of P4 conversion in the ovaries and testes of L. variegatus and indicates the rapid conversion of P4 into 5α-reduced metabolites in these tissues. Although P4 metabolism is not sex specific, sex-specific responses to P4 metabolites have been demonstrated previously. We hypothesize that the sex-specific responses of the ovaries and the testes to P4 may be associated with receptor-level regulatory processes.

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