Immunofluorescence in dermatology: A brief review

Immunofluorescence (IF) has been in use for the past five decades, both to investigate the pathophysiology of skin disorders and to help the dermatologists in the diagnosis of various bullous and connective tissue diseases. This review article, deals with different methods, applications, and recent advances in the IF methods used in dermatopathology. Here, we also discuss about the practical aspects of this technique such as handling of skin biopsy specimens and interpretation of direct and indirect IF findings.