介绍CELLBLOKS®:一种新颖的器官芯片平台,允许即插即用的方法来构建器官模型。

IF 2.4
In vitro models Pub Date : 2022-07-04 eCollection Date: 2022-12-01 DOI:10.1007/s44164-022-00027-8
Valon Llabjani, M R Siddique, Anaïs Macos, Afaf Abouzid, Valmira Hoti, Francis L Martin, Imran I Patel, Ahtasham Raza
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引用次数: 0

摘要

人体器官在结构和功能上都是复杂的系统。它们的功能是由许多特化细胞类型之间的相互作用驱动的,这很难在标准的培养皿格式上解开。传统的培养皿培养细胞的方法是静态的和自我限制的。然而,目前的器官芯片技术难以使用,吞吐量有限,缺乏与标准工作流程条件的兼容性。我们开发了CELLBLOKS®作为一种新型的“即插即用”器官芯片平台,可以直接创建多种细胞类型的器官特异性微环境。在这里,我们通过建立一个代表活体组织功能的肝脏模型来证明它的优势。CELLBLOKS®允许系统地测试和鉴定各种细胞组合,复制最佳的肝脏相关性。利用肝脏生化(CYP3A4和尿素)、细胞增殖指数和转运蛋白活性(白蛋白)分析成纤维细胞、内皮细胞和肝细胞的联合相互作用。结果表明,与传统的单一培养相比,利用共培养组合中的串扰可以实现最佳的肝功能。优化后的CELLBLOKS®肝脏模型使用他莫昔芬进行测试,分析药物诱导的肝毒性。数据表明,与单培养肝模型相比,我们的CELLBLOKS®肝模型对毒性损伤高度敏感。总之,CELLBLOKS®提供了一种新的细胞培养技术,用于创建与人类相关的器官型模型,这些模型易于在实验室环境中建立。补充信息:在线版本包含补充信息,获取地址:10.1007/s44164-022-00027-8。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Introducing CELLBLOKS®: a novel organ-on-a-chip platform allowing a plug-and-play approach towards building organotypic models.

Human organs are structurally and functionally complex systems. Their function is driven by the interactions between many specialised cell types, which is difficult to unravel on a standard Petri dish format. Conventional "Petri dish" approaches to culturing cells are static and self-limiting. However, current organ-on-a-chip technologies are difficult to use, have a limited throughput and lack compatibility with standard workflow conditions. We developed CELLBLOKS® as a novel "plug-and-play" organ-on-a-chip platform that enables straightforward creation of multiple cell-type organ-specific microenvironments. Herein, we demonstrate its advantages by building a liver model representative of live tissue function. CELLBLOKS® allows one to systematically test and identify various cell combinations that replicate optimal hepatic relevance. The combined interactions of fibroblasts, endothelial cells and hepatocytes were analysed using hepatic biochemistry (CYP3A4 and urea), cellular proliferation indices and transporter activities (albumin). The results demonstrate that optimal liver function can be achieved by exploiting crosstalk in co-culture combinations compared to conventional mono-culture. The optimised CELLBLOKS® liver model was tested to analyse drug-induced liver toxicity using tamoxifen. The data suggests that our CELLBLOKS® liver model is highly sensitive to toxic insult compared to mono-culture liver models. In summary, CELLBLOKS® provides a novel cell culture technology for creating human-relevant organotypic models that are easy and straightforward to establish in laboratory settings.

Supplementary information: The online version contains supplementary material available at 10.1007/s44164-022-00027-8.

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