从伊朗烧伤伤口分离的鲍曼不动杆菌中广谱β -内酰胺酶的流行

N. H. Jazani, H. Babazadeh, M. Sohrabpour, M. Zartoshti, M. Ghasemi-rad
{"title":"从伊朗烧伤伤口分离的鲍曼不动杆菌中广谱β -内酰胺酶的流行","authors":"N. H. Jazani, H. Babazadeh, M. Sohrabpour, M. Zartoshti, M. Ghasemi-rad","doi":"10.5580/1f87","DOIUrl":null,"url":null,"abstract":"Acinetobacter baumannii is a Gram-negative bacillus found in many hospital environments and its very high resistance to \nantimicrobial renders it as a successful nosocomial pathogen. There are many reports of Multi Drug Resistant A. baumannii \nfrom hospitals all over the world. ESBLs are beta-lactamases that hydrolyze extended-spectrum cephalosporins with an \noxyimino side chain; These cephalosporins include cefotaxime, ceftriaxone, and ceftazidime. The ESBLs are frequently plasmid \nencoded. Plasmids responsible for ESBL production frequently carry genes encoding resistance to other drug classes (for \nexample, aminoglycosides). Therefore, antibiotic options in the treatment of ESBL-producing organisms are extremely limited. \nThis study aimed to assess the incidence of ESBLs in 48 burn isolates of A. baumannii. The susceptibilities of isolates to \ndifferent antibiotics were tested by the Kirby-Bauer method. The Minimum inhibitory concentration of cefotaxime for each isolate \nwas determined by Hicomb strips in the range of 0.001-240 μg of antibiotic. For phenotypic detection of the ESBLs double disc \ndiffusion method. Cefazolin (100%), ciprofloxacin (100%) ofloxacin (95.8%) and kanamycin (95.8%) showed the highest rate of \nresistance and amikacin (52%) and imipenem (14.6%) demonstrated the lowest. 45.8% of isolates showed resistance to the 11 \ntested antimicrobials. 46 isolates (95.8%) were resistant to all tested concentrations of Cefotaxime (The Minimum inhibitory \nconcentrations were above 240 μg). only one isolate (2%) has been considered as ESBL producing isolate. The high resistance \nof isolates to cefotaxime, ceftazidime and cefepime in companion with the low number of ESBL producing isolates, proposed \nanother resistance mechanisms for these isolates to extended-spectrum cephalosporins.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2010-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"8","resultStr":"{\"title\":\"The prevalence of extended spectrum beta-lactamases in acinetobacter baumannii isolates from burn wounds in Iran\",\"authors\":\"N. H. Jazani, H. Babazadeh, M. Sohrabpour, M. Zartoshti, M. Ghasemi-rad\",\"doi\":\"10.5580/1f87\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Acinetobacter baumannii is a Gram-negative bacillus found in many hospital environments and its very high resistance to \\nantimicrobial renders it as a successful nosocomial pathogen. There are many reports of Multi Drug Resistant A. baumannii \\nfrom hospitals all over the world. ESBLs are beta-lactamases that hydrolyze extended-spectrum cephalosporins with an \\noxyimino side chain; These cephalosporins include cefotaxime, ceftriaxone, and ceftazidime. The ESBLs are frequently plasmid \\nencoded. Plasmids responsible for ESBL production frequently carry genes encoding resistance to other drug classes (for \\nexample, aminoglycosides). Therefore, antibiotic options in the treatment of ESBL-producing organisms are extremely limited. \\nThis study aimed to assess the incidence of ESBLs in 48 burn isolates of A. baumannii. The susceptibilities of isolates to \\ndifferent antibiotics were tested by the Kirby-Bauer method. The Minimum inhibitory concentration of cefotaxime for each isolate \\nwas determined by Hicomb strips in the range of 0.001-240 μg of antibiotic. For phenotypic detection of the ESBLs double disc \\ndiffusion method. Cefazolin (100%), ciprofloxacin (100%) ofloxacin (95.8%) and kanamycin (95.8%) showed the highest rate of \\nresistance and amikacin (52%) and imipenem (14.6%) demonstrated the lowest. 45.8% of isolates showed resistance to the 11 \\ntested antimicrobials. 46 isolates (95.8%) were resistant to all tested concentrations of Cefotaxime (The Minimum inhibitory \\nconcentrations were above 240 μg). only one isolate (2%) has been considered as ESBL producing isolate. The high resistance \\nof isolates to cefotaxime, ceftazidime and cefepime in companion with the low number of ESBL producing isolates, proposed \\nanother resistance mechanisms for these isolates to extended-spectrum cephalosporins.\",\"PeriodicalId\":22514,\"journal\":{\"name\":\"The Internet journal of microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2010-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Internet journal of microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5580/1f87\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Internet journal of microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5580/1f87","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 8

摘要

鲍曼不动杆菌是在许多医院环境中发现的革兰氏阴性杆菌,其对抗菌素的高耐药性使其成为一种成功的医院病原体。世界各地的医院都有许多关于多重耐药鲍曼不动杆菌的报道。ESBLs是β -内酰胺酶,可水解具有氧亚胺侧链的广谱头孢菌素;这些头孢菌素包括头孢噻肟、头孢曲松和头孢他啶。esbl通常是质粒编码的。负责产生ESBL的质粒经常携带对其他药物类(例如氨基糖苷类)产生抗性的基因。因此,抗生素的选择在治疗产生esbl的生物体是极其有限的。本研究旨在评估48株鲍曼不动杆菌烧伤分离株中ESBLs的发生率。采用Kirby-Bauer法测定菌株对不同抗生素的敏感性。采用Hicomb试纸测定各菌株对头孢噻肟的最低抑菌浓度在0.001 ~ 240 μg抗生素范围内。用于ESBLs表型检测的双盘扩散法。头孢唑林(100%)、环丙沙星(100%)、氧氟沙星(95.8%)和卡那霉素(95.8%)的耐药率最高,阿米卡星(52%)和亚胺培南(14.6%)的耐药率最低。45.8%的分离株对11种抗微生物药物有耐药性。46株(95.8%)对头孢噻肟耐药,最低抑菌浓度均在240 μg以上。只有一个分离株(2%)被认为是产生ESBL的分离株。分离株对头孢噻肟、头孢他啶和头孢吡肟具有高耐药性,同时产生ESBL的分离株数量较少,这可能是这些分离株对广谱头孢菌素的另一种耐药机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The prevalence of extended spectrum beta-lactamases in acinetobacter baumannii isolates from burn wounds in Iran
Acinetobacter baumannii is a Gram-negative bacillus found in many hospital environments and its very high resistance to antimicrobial renders it as a successful nosocomial pathogen. There are many reports of Multi Drug Resistant A. baumannii from hospitals all over the world. ESBLs are beta-lactamases that hydrolyze extended-spectrum cephalosporins with an oxyimino side chain; These cephalosporins include cefotaxime, ceftriaxone, and ceftazidime. The ESBLs are frequently plasmid encoded. Plasmids responsible for ESBL production frequently carry genes encoding resistance to other drug classes (for example, aminoglycosides). Therefore, antibiotic options in the treatment of ESBL-producing organisms are extremely limited. This study aimed to assess the incidence of ESBLs in 48 burn isolates of A. baumannii. The susceptibilities of isolates to different antibiotics were tested by the Kirby-Bauer method. The Minimum inhibitory concentration of cefotaxime for each isolate was determined by Hicomb strips in the range of 0.001-240 μg of antibiotic. For phenotypic detection of the ESBLs double disc diffusion method. Cefazolin (100%), ciprofloxacin (100%) ofloxacin (95.8%) and kanamycin (95.8%) showed the highest rate of resistance and amikacin (52%) and imipenem (14.6%) demonstrated the lowest. 45.8% of isolates showed resistance to the 11 tested antimicrobials. 46 isolates (95.8%) were resistant to all tested concentrations of Cefotaxime (The Minimum inhibitory concentrations were above 240 μg). only one isolate (2%) has been considered as ESBL producing isolate. The high resistance of isolates to cefotaxime, ceftazidime and cefepime in companion with the low number of ESBL producing isolates, proposed another resistance mechanisms for these isolates to extended-spectrum cephalosporins.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信