蛋黄和椰奶增稠剂对尼日利亚迈杜古里冷藏和冷冻火鸡精液质量的影响

S. Asuku, I. Alkali, M. M. Bukar, M. Waziri, A. Mustapha, A. Y. Ribadu, J. D. Amin
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引用次数: 0

摘要

本研究研究了蛋黄和椰奶基膨化剂对尼日利亚迈杜古里冷藏和解冻后火鸡精液活力和形态的影响。采用背腹按摩法,每周2次采集7只成年火鸡的精液样本112份,连续2个月。采集新鲜精液,分成三等分,分别用改良林格氏缓冲液、蛋黄和椰奶填充剂稀释。每个延长的精液分别在37°C和5°C保存,然后每小时评估一次,持续24小时。延长的精液样本也在液氮中冷冻保存,解冻后的质量在24小时后进行评估。精子在37°C下可保持6小时的进行性运动,在5°C下保存在椰奶和蛋黄为基础的扩展剂中可保持12小时。还发现,使用1%椰奶或1%蛋黄为基础的扩展剂可以改善火鸡精液在-196°C冷冻后的解冻后质量。结果表明,以椰奶为基础的膨化剂比以蛋黄为基础的膨化剂更能保持精子活力,减少精子异常。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Egg Yolk and Coconut Milk-Based Extenders on Chilled and Cryopreserved Turkey (Meleagris gallopavo) Semen Qualities in Maiduguri, Nigeria
This study investigated the effects of egg yolk and coconut milk-based extenders on the motility and morphology of chilled and post thawed turkey (Meleagris gallopavo) semen in Maiduguri, Nigeria. A total of 112 semen samples were collected from seven matured turkey toms, twice weekly for 2 months using the dorso-abdominal massage method. Freshly collected semen were pooled, divided into three aliquots and diluted with modified Ringer’s buffer, egg yolk and coconut milk-based extenders, respectively. An aliquot of each extended semen was stored at 37°C and 5°C, and then evaluated hourly for 24 hours. Extended semen samples were also cryopreserved in liquid nitrogen and post thaw qualities were assessed after 24 hours. The progressive motility of spermatozoa was maintained for 6 hours at 37°C and up to 12 hours at 5°C when preserved in coconut milk and egg yolk-based extenders. It was also found that use of 1% coconut milk or 1% egg yolk-based extenders improved the post thaw quality of turkey semen once frozen at -196°C. It was observed that the coconut milk-based extender preserved the spermatozoa motility and reduced sperm abnormalities better than the egg yolk-based extender.
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