HPLC DETECTION OF SERUM AND URINARY PARAPHENYLENE DIAMINE (PPD) LEVEL AND ITS METABOLITES IN RELATION TO CARDIAC TOXICITY

Hair dye poisoning is a significant emerging problem in Upper Egypt. The main component of hair dye causing toxicity is para-phenylenediamine (PPD). Ingestion of PPD could be accidental or suicidal. After oral intake, it is metabolized in the liver into N-mono-(MAPPD) and N, N′- diacetylated (DAPPD) metabolites. Tissue damage after PPD ingestions occurs due to increased free radical and oxidative stress that depletes tissue glutathione. Cardiac toxicity is a complication of PPD poisoning. It could be manifested by hypotension, different arrhythmia; besides, myocarditis and elevated cardiac biomarkers were also found. Serum and urinary levels of PPD, N-acetyl-p-phenylenediamine (MAPPD), and N--diacetyl-p-phenylenediamine (DAPPD) were measured by HPLC. A prospective cohort study was planned to determine the relationship between the serum and urinary PPD, N-acetyl-p-phenylenediamine (MAPPD) and N, on -diacetyl-p-phenylenediamine (DAPPD) levels with cardiac manifestations of the poisoned patient. Forty patients completed the diagnosis to have acute poisoning following hair dye ingestion. PPD and its metabolite concentrations did not show any significant correlation with the prevalence of cardiac toxicity and could not predict its occurrence among studied patient (p-value< .05)