利用分子标记技术筛选伊朗甜瓜地方品种抗枯萎病

Atefeh Gholizadegan, A. Seifi
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引用次数: 0

摘要

枯萎病是甜瓜(Cucumis melo L.)最具破坏性的病害之一,是世界范围内造成甜瓜种植区产量损失的重要经济病害。利用抗性基因对宿主进行抗性是防治枯萎病最有效的措施之一。利用开发的对甜瓜镰刀菌1小种具有抗性的形式-2基因分子标记,对伊朗霍拉桑-拉扎维地区甜瓜地方品种进行了筛选。在一组不同的抗性和易感品系上对标记进行验证后,我们确定STS312标记为多态且易于标记。然后,我们使用STS312对5个不同地方品种的植物进行基因型分析。结果表明,在两个地方品种Eyvankey和Mashhadi中均存在form -2抗性等位基因。这些地方品种可用于甜瓜育种,以提高甜瓜对枯萎病的抗性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Screening some Iranian Muskmelon Landraces for Resistance Against Fusarium Wilt Disease using Molecular Markers
Fusarium wilt is one of the most destructive diseases of muskmelon (Cucumis melo L.), which is an economically important disease worldwide causes yield losses in muskmelon growing areas. One of the most effective controlling measures to prevent Fusarium wilt is through host resistance by using resistance genes. We used developed molecular markers for Fom-2 gene, which confers resistance to race 1 of Fusarium in muskmelon, to screen muskmelon landraces in Khorasane-e-Razavi, Iran. After validation of the markers on a differential set of resistant and susceptible lines, we identified STS312 marker as the polymorphic and easy-to-score marker. Then we used STS312 to genotype plants from five different landraces. Our results suggest that resistance allele of Fom-2 gene is present in two landraces: Eyvankey and Mashhadi. These landraces can be used by muskmelon breeders to enhance resistance to Fusarium wilt in muskmelon.
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