印度米佐拉姆邦Aizawl地区生姜软腐病病原真菌的分离与分子鉴定

G. Rosangkima, Vanramliana, H. Lalthanzara, Lalringngheti, H. Lalramnghaki
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引用次数: 4

摘要

由真菌病原菌引起的生姜软腐病已成为世界范围内导致生姜减产的最严重问题之一。它还在印度米佐拉姆邦的农民中造成了一个重大问题,导致根茎产量大幅下降。然而,这种疾病的确切病原体尚未在该州确定。因此,本研究从印度米佐拉姆邦Aizawl地区5个不同村庄的生姜病株中分离和鉴定了姜软腐病的病原。对分离真菌进行培养,利用rDNA内转录间隔段进行形态学和分子鉴定。在5个村的生姜样品中检出尖孢镰刀菌、茄枯镰刀菌和黄瓜枯萎镰刀菌。镰刀菌是最常见的,似乎是主要的病原体。建议对全国生姜软腐病病原真菌的多样性进行深入调查,为制定有效的生态友好型病害管理方案提供依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and molecular characterization of ginger soft rot pathogenic fungi in Aizawl district of Mizoram, India
Ginger soft rot disease caused by fungal pathogens have become one of the most serious problems causing reduced production around the world. It has also caused a major problem among farmers of Mizoram state in India resulting in a huge decline in rhizome yield. However, the exact causative agents of this disease have not been identified in the state. Therefore, the present study was undertaken to isolate and characterized the causative agents of ginger soft rot disease from the diseased plants collected from five different villages of Aizawl district, Mizoram. Isolated fungi were cultured and morphological and molecular identification were done using internal transcribed spacer of rDNA. Fusarium oxysporum, F. solani and Plectosphaerella cucumerina were identified in ginger samples from five villages. Fusarium spp. were the most common and seem to be the major causative agents. It is suggested that further investigation is required to explore the diversity of ginger soft rot pathogenic fungi in the whole state which could be helpful in introducing effective and eco-friendly disease management programme.
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