一种可用于比色细胞活力测定的水溶性四氮唑盐

Analytical Communications Pub Date : 1999-01-01 DOI:10.1039/A809656B

H. Tominaga, M. Ishiyama, Fumio Ohseto, K. Sasamoto, T. Hamamoto, Keiji Suzuki, M. Watanabe

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引用次数: 584

摘要

描述了一种四氮唑盐，2-(2-甲氧基-4-硝基苯基)-3-(4-硝基苯基)-5-(2,4-二硫苯基)- 2h四氮唑单钠盐(WST-8)在细胞活力测定和体外药敏试验中的应用。与传统四氮唑盐相比，WST-8作为细胞活力的显色指标具有更高的灵敏度，其细胞活力和IC50值分别与MTT法和[3H]胸苷摄取法吻合良好。

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A water-soluble tetrazolium salt useful for colorimetric cell viability assay

The application of a tetrazolium salt, 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium, monosodium salt (WST-8), to cell viability assays and in vitro drug sensitivity tests is described. With a higher sensitivity as a chromogenic indicator for cell viability compared with conventional tetrazolium salts, WST-8 produced results of cell viability and IC50 values that were in good agreement, respectively, with the MTT method and [3H]thymidine uptake method.

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Analytical Communications

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