停留时间对连续反应器中ScCO2分离乳清蛋白的影响

Q3 Chemical Engineering

Chemical engineering transactions Pub Date : 2021-07-01 DOI:10.3303/CET2187090

P. P. D. Souza, J. C. Lima, L. Cardozo-Filho, F. Seixas, V. F. Cabral

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引用次数: 0

摘要

本研究提出在连续反应器中使用乳清分离蛋白(WPI)和超临界二氧化碳(scCO2)溶液(5%)对蛋白质进行分馏。实验在不同的压力、温度和停留时间下进行，分别为65.4、34.3和32.3 min。采用高效液相色谱法测定a-乳清蛋白(a-LA)和s-乳球蛋白(s-LG)水平。未经处理的WPI显示a/s比= 0.40。16 MPa/55°C和16 MPa/60°C分别是对停留时间增加和减少影响最大的正、负条件。在24 MPa, 65℃，4.0 mL·min-1的最严酷条件下，圆二色性表明，尽管没有获得高纯度，但采用scCO2的分离技术并未使蛋白质a- la和s-LG变性。

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Effect of Residence Time on the Fractionation of Whey Protein Isolate with ScCO2 in a Continuous Reactor

This study proposes a fractionation of proteins using solutions (5%) of Whey Protein Isolate (WPI) and supercritical carbon dioxide (scCO2) in a continuous reactor. The experiments were performed at different values of pressure, temperature, and residence times - 65.4, 34.3, and 32.3 min, respectively. a-lactalbumin (a-LA) and s-lactoglobulin (s-LG) levels were determined by HPLC. WPI used without treatment showed an a/s ratio = 0.40. 16 MPa/55 °C and 16 MPa/60 °C were the conditions that suffered the most positive and negative influence of the increase and decrease in residence time, respectively. In the most severe condition, 24 MPa, 65 °C, and 4.0 mL·min-1, circular dichroism indicated that, despite not having obtained a high level of pureness, the fractionation technique employing scCO2 did not denature the proteins a-LA and s-LG.

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来源期刊

Chemical engineering transactions Chemical Engineering-Chemical Engineering (all)

CiteScore

1.40

自引率

0.00%

发文量

审稿时长

6 weeks

期刊介绍： Chemical Engineering Transactions (CET) aims to be a leading international journal for publication of original research and review articles in chemical, process, and environmental engineering. CET begin in 2002 as a vehicle for publication of high-quality papers in chemical engineering, connected with leading international conferences. In 2014, CET opened a new era as an internationally-recognised journal. Articles containing original research results, covering any aspect from molecular phenomena through to industrial case studies and design, with a strong influence of chemical engineering methodologies and ethos are particularly welcome. We encourage state-of-the-art contributions relating to the future of industrial processing, sustainable design, as well as transdisciplinary research that goes beyond the conventional bounds of chemical engineering. Short reviews on hot topics, emerging technologies, and other areas of high interest should highlight unsolved challenges and provide clear directions for future research. The journal publishes periodically with approximately 6 volumes per year. Core topic areas: -Batch processing- Biotechnology- Circular economy and integration- Environmental engineering- Fluid flow and fluid mechanics- Green materials and processing- Heat and mass transfer- Innovation engineering- Life cycle analysis and optimisation- Modelling and simulation- Operations and supply chain management- Particle technology- Process dynamics, flexibility, and control- Process integration and design- Process intensification and optimisation- Process safety- Product development- Reaction engineering- Renewable energy- Separation processes- Smart industry, city, and agriculture- Sustainability- Systems engineering- Thermodynamic- Waste minimisation, processing and management- Water and wastewater engineering