利用大豆乳清生产生物纤维素的Komagataeibacter sp. PAP1培养条件的统计优化

IF 0.7 4区综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES

Maejo International Journal of Science and Technology Pub Date : 2014-01-01 DOI:10.14456/MIJST.2014.1

A. Suwanposri, P. Yukphan, Yuzo Yamada, D. Ochaikul

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引用次数: 30

摘要

以农业副产品大豆乳清(SBW)为原料制备了一种低成本培养基，并用Komagataeibacter sp. PAP1对其进行了优化，用于生产生物纤维素(BC)。产BC的最佳条件为:pH 6.21，乙醇浓度(v/v) 1.61%， 28.4℃。与标准Hestrin-Schramm (HS)培养基相比，使用SBW优化培养基可使BC产量增加3.6倍。与从标准HS介质中产生的膜相比，从优化介质中产生的BC膜更强，更不渗透水蒸气和氧气。这些特性使SBW薄膜发展成为氧气敏感产品的环保食品包装。它的高吸水能力使其在生物医学应用中使用，如伤口敷料。我们的研究结果表明，SBW可以作为一种低成本的衬底用于商业规模的BC生产。

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Statistical optimisation of culture conditions for biocellulose production by Komagataeibacter sp. PAP1 using soya bean whey

A low-cost medium based on soya bean whey (SBW) was prepared as a by-product from agriculture and optimised for biocellulose (BC) production by Komagataeibacter sp. PAP1. The optimal conditions for BC production are: pH 6.21, 1.61% ethanol concentration (v/v) and 28.4 o C. The use of optimised medium based on SBW increased BC production 3.6 fold compared to standard Hestrin-Schramm (HS) medium. The BC film produced from the optimised medium was stronger and more impermeable to water vapour and oxygen compared to the one produced from the standard HS medium. These properties allow SBW film to be developed into eco-friendly food packaging for oxygen-sensitive products. Its high water absorption capacity allows its use in biomedical application such as wound dressing. Our results demonstrate that SBW can be used as an alternative low-cost substrate for BC production on commercial scale.

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来源期刊

Maejo International Journal of Science and Technology 综合性期刊-综合性期刊

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

15 months

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