含马龙花提取物水凝胶抗炎活性的初步筛选

A. Jain
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引用次数: 1

摘要

目的:通过不同类型的动物模型,初步评价马龙花甲醇部分水凝胶制剂的抗炎作用。材料与方法:以不同比例的卡波波尔940和羧甲基纤维素钠(CMC)为原料,按标准方法制备了5个含提取物的水凝胶配方和1个不含提取物的对照。对制备的水凝胶进行了优化表征,并对二甲苯致小鼠耳水肿和巴豆油致小鼠耳水肿进行了抗炎活性筛选。通过测量耳水肿抑制百分比和生化参数,如一氧化氮水平和髓过氧化物酶(MPO)水平,观察效果。结果:植物化学筛选表明,黄花楸石油醚提取物中含有甾醇类、萜类和脂肪油,而黄花楸乙醇提取物中含有苷类、酚类化合物、黄酮类和氨基酸。其他评价参数如pH、黏度、铺展性和稠度均以caropol 940用量为1.5 g、CMC钠用量为1%为最佳释放条件。MAMFH治疗组的水肿抑制率(66.67%)高于标准治疗组(65.59%)。结果显示,与非炎症对照组和水凝胶制剂相比,炎症对照组和仅用凝胶基质处理的耳部MPO水平显著升高(P < 0.01)。结果显示,巴豆油诱导中性粒细胞流入小鼠耳组织的能力。水凝胶提取物和伏他仑凝胶可显著降低小鼠耳中MPO水平(P < 0.05)。结论:黄花楸甲醇组分对二甲苯致耳部水肿有一定的抑制作用。这可能是由于提取物能够抑制二甲苯的合成、释放或参与炎症的作用。甲醇提取物通过抑制组胺的合成、释放或抑制组胺的作用而发挥抗炎作用。黄花楸叶甲醇提取物中黄酮类化合物的存在可能是黄酮类化合物活性显著的原因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Preliminary screening of hydrogel containing Martynia annua extract for anti-inflammatory activity
Objective: Aim of the present study was to investigate preliminary evaluation of hydrogel formulation of methanol fraction of Martynia annua for anti-inflammatory activity using different types of animal models. Materials and Methods: Five different hydrogel formulations containing extract and one control without extract were prepared according to standard method using different proportions of Carbopol 940 and sodium carboxymethyl cellulose (CMC). Prepared hydrogel was characterized for optimization and screened for anti-inflammatory activity using Xylene induced ear edema and Croton oil-induced ear edema in mice. Effect was observed by measurement of percent inhibition of ear edema and biochemical parameters, for example, nitric oxide level and myeloperoxidase (MPO) level. Results: Phytochemical screening showed that petroleum ether extract of M. annua showed the presence of sterols, terpenoids, and fatty oils while ethanol extract of showed the presence of glycosides, phenolic compounds, flavonoids, and amino acids. Results of all other evaluation parameters, for example, pH, viscosity, spreadability, and consistency were found suitable in optimized formulation as 1.5 g of Carbopol 940 and 1% of Sodium CMC for best drug release profile. Effect of MAMFH was observed greater percentage of edema inhibition (66.67%) and was comparable to standard group of treatment (65.59%). Results showed that MPO level of inflamed ears (inflamed control and treated only with gel base) was significantly higher in comparison with the non-inflamed control (P < 0.01), and with hydrogel formulations. The results showing the ability of croton oil to induce neutrophil influx into mice ear tissue. This may be reduced by treatment with extract hydrogels, as well as Voltaren Emulgel, reduced significantly (P < 0.05) the MPO levels in mice ears. Conclusion: Methanol fraction of M. annua was capable of inhibiting ear edema induced by xylene. It may be due to the ability of extract to either inhibit the synthesis, release or action of xylene involved in the inflammation. Methanol extracts was exhibits its anti-inflammatory action by inhibiting the synthesis, release or action of histamine. Significant activity may be due to presence of flavonoids in methanol extract fraction of M. annua leaves.
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