Therapeutic Effects of Medical Ozone in the Functions and Histopathological Features of the Ovary in an Experimental Torsion-Detorsion Model

Background: Ovarian torsion is a serious gynecological condition, encountered especially during reproductive age. Medical ozone has therapeutic effects with its antioxidant, apoptotic, and anti-inflammatory properties. Objective: This study aimed to determine the effects of medical ozone on the ovarian functions and ovarian morphology in an experimental rat ovarian torsion-detorsion model. Methods: Twenty female Wistar albino rats were used in this study. The rats were randomized into two groups: Group 1 (torsion/detorsion+ozone) (n=10) and Group 2 (only torsion/detorsion) (n=10). Histopathological evaluation of the ovarian tissue was performed. Additionally, Total Antioxidant Capacity (TAC), Follicle-Stimulating Hormone (FSH), Estradiol (E2), and Lactate Dehydrogenase (LDH) levels were measured in the serum. Results: Ozone application has to lead to a decrease in histopathological parameters such as hemorrhage, vascular congestion, cellular apoptosis, and necrosis while no histopathological changes were detected concerning edema and inflammatory cells. High TAC in Group 1 and high FSH in Group 2 were detected. A statistically significant difference between the two groups was detected in terms of TAC (p=0.001) and FSH (p=0.002). There were no significant differences between Group1 and Group2 regarding E2 (p=0.757), and LDH levels (p=0.453). Conclusion: edical ozone is shown to positively affect the histopathological markers of cellular damage and increase the antioxidant capacity in the torsion-detorsion model. We suggest that further animal model studies may be designed to reveal the factors behind the effect of medical ozone.