GFP和Cre重组酶表达小鼠迁移皮层神经元的实时记录

Q2 Neuroscience
Sylvia Tielens, Juliette D. Godin, Laurent Nguyen
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引用次数: 9

摘要

大脑皮层是大脑中最复杂的区域之一,其发育需要复杂的细胞迁移模式。实验观察表明,投射神经元在皮层壁内呈放射状迁移,而中间神经元则沿着多条切向路径迁移,到达发育中的皮层。对细胞迁移过程的严格调控确保了神经元与特定大脑皮层回路的正确定位和功能整合。神经元迁移的中断通常会导致皮质功能障碍和/或与神经系统疾病相关的畸形。因此,揭示神经元迁移的分子控制对于理解大脑皮层的生理或病理发展至关重要。在本单元中,提出了允许详细分析中间神经元和投射神经元在不同实验条件下(即功能丧失或获得)迁移模式的协议。©2016,作者:John Wiley&;股份有限公司。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Real-time Recordings of Migrating Cortical Neurons from GFP and Cre Recombinase Expressing Mice

The cerebral cortex is one of the most intricate regions of the brain that requires elaborate cell migration patterns for its development. Experimental observations show that projection neurons migrate radially within the cortical wall, whereas interneurons migrate along multiple tangential paths to reach the developing cortex. Tight regulation of the cell migration processes ensures proper positioning and functional integration of neurons to specific cerebral cortical circuits. Disruption of neuronal migration often leads to cortical dysfunction and/or malformation associated with neurological disorders. Unveiling the molecular control of neuron migration is thus fundamental to understanding the physiological or pathological development of the cerebral cortex. In this unit, protocols allowing detailed analysis of patterns of migration of both interneurons and projection neurons under different experimental conditions (i.e., loss or gain of function) are presented. © 2016 by John Wiley & Sons, Inc.

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来源期刊
Current Protocols in Neuroscience
Current Protocols in Neuroscience Neuroscience-Neuroscience (all)
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期刊介绍: Current Protocols in Neuroscience is a one-stop resource for finding and adapting the best models and methods for all types of neuroscience experiments. Updated every three months in all formats, CPNS is constantly evolving to keep pace with the very latest discoveries and developments. A year of these quarterly updates is included in the initial CPNS purchase price.
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