Juana Pincheira , Mireya Bravo , Manuel J Santos , Consuelo de la Torre , Jorge F López-Sáez
{"title":"范科尼贫血淋巴细胞：dl-α-生育酚（维生素E）对染色单体断裂和G2修复效率的影响","authors":"Juana Pincheira , Mireya Bravo , Manuel J Santos , Consuelo de la Torre , Jorge F López-Sáez","doi":"10.1016/S0921-8777(00)00058-6","DOIUrl":null,"url":null,"abstract":"<div><p>The high frequency of chromosomal breaks in Fanconi anemia (FA) lymphocytes has been related to the increased oxidative damage shown by these cells.</p><p>The effect of 100<!--> <!-->μM <span>dl</span>-α-tocopherol (Vitamin E) on the level of chromosomal damage in mitosis was studied in lymphocytes from five FA patients and from age matched controls, both under basal conditions and when G<sub>2</sub> repair was prevented by 2.5<!--> <!-->mM caffeine (G<sub>2</sub> unrepaired damage). In addition, the effect of this antioxidant on G<sub>2</sub> duration and the efficiency of G<sub>2</sub> repair was also evaluated in the sample.</p><p>α-Tocopherol (AT) decreased the frequency of chromosomal damage (under basal and inhibited G<sub>2</sub> repair conditions) and the duration of G<sub>2</sub><span> in FA cells. This antioxidant protective effect, expressed as the decrease in chromatid breaks, was greater in FA cells (50.8%) than in controls (25%).</span></p><p>The efficiency of the G<sub>2</sub> repair process (G<sub>2</sub>R rate) defined as the ratio between the percentage of chromatid breaks repaired in G<sub>2</sub><span> and the duration of this cell cycle phase was lesser in FA cells (10.6) than in controls (22.6). AT treatment slightly increased this G</span><sub>2</sub>R rate, both in FA cells and controls.</p><p>These results suggest that an increased oxidative damage and a lower G<sub>2</sub> repair rate may be simultaneously involved in the high frequency of chromatid damage detected in FA cells.</p></div>","PeriodicalId":100935,"journal":{"name":"Mutation Research/DNA Repair","volume":"461 4","pages":"Pages 265-271"},"PeriodicalIF":0.0000,"publicationDate":"2001-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0921-8777(00)00058-6","citationCount":"11","resultStr":"{\"title\":\"Fanconi anemia lymphocytes: effect of dl-α-tocopherol (Vitamin E) on chromatid breaks and on G2 repair efficiency\",\"authors\":\"Juana Pincheira , Mireya Bravo , Manuel J Santos , Consuelo de la Torre , Jorge F López-Sáez\",\"doi\":\"10.1016/S0921-8777(00)00058-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The high frequency of chromosomal breaks in Fanconi anemia (FA) lymphocytes has been related to the increased oxidative damage shown by these cells.</p><p>The effect of 100<!--> <!-->μM <span>dl</span>-α-tocopherol (Vitamin E) on the level of chromosomal damage in mitosis was studied in lymphocytes from five FA patients and from age matched controls, both under basal conditions and when G<sub>2</sub> repair was prevented by 2.5<!--> <!-->mM caffeine (G<sub>2</sub> unrepaired damage). In addition, the effect of this antioxidant on G<sub>2</sub> duration and the efficiency of G<sub>2</sub> repair was also evaluated in the sample.</p><p>α-Tocopherol (AT) decreased the frequency of chromosomal damage (under basal and inhibited G<sub>2</sub> repair conditions) and the duration of G<sub>2</sub><span> in FA cells. This antioxidant protective effect, expressed as the decrease in chromatid breaks, was greater in FA cells (50.8%) than in controls (25%).</span></p><p>The efficiency of the G<sub>2</sub> repair process (G<sub>2</sub>R rate) defined as the ratio between the percentage of chromatid breaks repaired in G<sub>2</sub><span> and the duration of this cell cycle phase was lesser in FA cells (10.6) than in controls (22.6). AT treatment slightly increased this G</span><sub>2</sub>R rate, both in FA cells and controls.</p><p>These results suggest that an increased oxidative damage and a lower G<sub>2</sub> repair rate may be simultaneously involved in the high frequency of chromatid damage detected in FA cells.</p></div>\",\"PeriodicalId\":100935,\"journal\":{\"name\":\"Mutation Research/DNA Repair\",\"volume\":\"461 4\",\"pages\":\"Pages 265-271\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2001-01-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0921-8777(00)00058-6\",\"citationCount\":\"11\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mutation Research/DNA Repair\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0921877700000586\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mutation Research/DNA Repair","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0921877700000586","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Fanconi anemia lymphocytes: effect of dl-α-tocopherol (Vitamin E) on chromatid breaks and on G2 repair efficiency
The high frequency of chromosomal breaks in Fanconi anemia (FA) lymphocytes has been related to the increased oxidative damage shown by these cells.
The effect of 100 μM dl-α-tocopherol (Vitamin E) on the level of chromosomal damage in mitosis was studied in lymphocytes from five FA patients and from age matched controls, both under basal conditions and when G2 repair was prevented by 2.5 mM caffeine (G2 unrepaired damage). In addition, the effect of this antioxidant on G2 duration and the efficiency of G2 repair was also evaluated in the sample.
α-Tocopherol (AT) decreased the frequency of chromosomal damage (under basal and inhibited G2 repair conditions) and the duration of G2 in FA cells. This antioxidant protective effect, expressed as the decrease in chromatid breaks, was greater in FA cells (50.8%) than in controls (25%).
The efficiency of the G2 repair process (G2R rate) defined as the ratio between the percentage of chromatid breaks repaired in G2 and the duration of this cell cycle phase was lesser in FA cells (10.6) than in controls (22.6). AT treatment slightly increased this G2R rate, both in FA cells and controls.
These results suggest that an increased oxidative damage and a lower G2 repair rate may be simultaneously involved in the high frequency of chromatid damage detected in FA cells.
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