Summer N. Horchler, Patrick C. Hancock, Mingjie Sun, Alexander T. Liu, Sameer Massand, Jessica C. El-Mallah, Dana Goldenberg, Olivia Waldron, Mary E. Landmesser, Shailaja Agrawal, Srinivas V. Koduru, Dino J. Ravnic
{"title":"精密微穿刺后的血管持久性。","authors":"Summer N. Horchler, Patrick C. Hancock, Mingjie Sun, Alexander T. Liu, Sameer Massand, Jessica C. El-Mallah, Dana Goldenberg, Olivia Waldron, Mary E. Landmesser, Shailaja Agrawal, Srinivas V. Koduru, Dino J. Ravnic","doi":"10.1111/micc.12835","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objective</h3>\n \n <p>The success of engineered tissues continues to be limited by time to vascularization and perfusion. Recently, we described a simple microsurgical approach, termed micropuncture (MP), which could be used to rapidly vascularize an adjacently placed scaffold from the recipient macrovasculature. Here we studied the long-term persistence of the MP-induced microvasculature.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Segmental 60 μm diameter MPs were created in the recipient rat femoral artery and vein followed by coverage with a simple Type 1 collagen scaffold. The recipient vasculature and scaffold were then wrapped <i>en bloc</i> with a silicone sheet to isolate intrinsic vascularization. Scaffolds were harvested at 28 days post-implantation for detailed analysis, including using a novel artificial intelligence (AI) approach.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>MP scaffolds demonstrated a sustained increase of vascular density compared to internal non-MP control scaffolds (<i>p</i> < 0.05) secondary to increases in both vessel diameters (<i>p</i> < 0.05) and branch counts (<i>p</i> < 0.05). MP scaffolds also demonstrated statistically significant increases in red blood cell (RBC) perfused lumens.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>This study further highlights that the intrinsic MP-induced vasculature continues to persist long-term. Its combination of rapid and stable angiogenesis represents a novel surgical platform for engineered scaffold and graft perfusion.</p>\n </section>\n </div>","PeriodicalId":18459,"journal":{"name":"Microcirculation","volume":"31 1","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/micc.12835","citationCount":"0","resultStr":"{\"title\":\"Vascular persistence following precision micropuncture\",\"authors\":\"Summer N. Horchler, Patrick C. Hancock, Mingjie Sun, Alexander T. Liu, Sameer Massand, Jessica C. El-Mallah, Dana Goldenberg, Olivia Waldron, Mary E. Landmesser, Shailaja Agrawal, Srinivas V. Koduru, Dino J. Ravnic\",\"doi\":\"10.1111/micc.12835\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Objective</h3>\\n \\n <p>The success of engineered tissues continues to be limited by time to vascularization and perfusion. Recently, we described a simple microsurgical approach, termed micropuncture (MP), which could be used to rapidly vascularize an adjacently placed scaffold from the recipient macrovasculature. Here we studied the long-term persistence of the MP-induced microvasculature.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Segmental 60 μm diameter MPs were created in the recipient rat femoral artery and vein followed by coverage with a simple Type 1 collagen scaffold. The recipient vasculature and scaffold were then wrapped <i>en bloc</i> with a silicone sheet to isolate intrinsic vascularization. Scaffolds were harvested at 28 days post-implantation for detailed analysis, including using a novel artificial intelligence (AI) approach.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>MP scaffolds demonstrated a sustained increase of vascular density compared to internal non-MP control scaffolds (<i>p</i> < 0.05) secondary to increases in both vessel diameters (<i>p</i> < 0.05) and branch counts (<i>p</i> < 0.05). MP scaffolds also demonstrated statistically significant increases in red blood cell (RBC) perfused lumens.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>This study further highlights that the intrinsic MP-induced vasculature continues to persist long-term. 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Vascular persistence following precision micropuncture
Objective
The success of engineered tissues continues to be limited by time to vascularization and perfusion. Recently, we described a simple microsurgical approach, termed micropuncture (MP), which could be used to rapidly vascularize an adjacently placed scaffold from the recipient macrovasculature. Here we studied the long-term persistence of the MP-induced microvasculature.
Methods
Segmental 60 μm diameter MPs were created in the recipient rat femoral artery and vein followed by coverage with a simple Type 1 collagen scaffold. The recipient vasculature and scaffold were then wrapped en bloc with a silicone sheet to isolate intrinsic vascularization. Scaffolds were harvested at 28 days post-implantation for detailed analysis, including using a novel artificial intelligence (AI) approach.
Results
MP scaffolds demonstrated a sustained increase of vascular density compared to internal non-MP control scaffolds (p < 0.05) secondary to increases in both vessel diameters (p < 0.05) and branch counts (p < 0.05). MP scaffolds also demonstrated statistically significant increases in red blood cell (RBC) perfused lumens.
Conclusions
This study further highlights that the intrinsic MP-induced vasculature continues to persist long-term. Its combination of rapid and stable angiogenesis represents a novel surgical platform for engineered scaffold and graft perfusion.
期刊介绍：
The journal features original contributions that are the result of investigations contributing significant new information relating to the vascular and lymphatic microcirculation addressed at the intact animal, organ, cellular, or molecular level. Papers describe applications of the methods of physiology, biophysics, bioengineering, genetics, cell biology, biochemistry, and molecular biology to problems in microcirculation.
Microcirculation also publishes state-of-the-art reviews that address frontier areas or new advances in technology in the fields of microcirculatory disease and function. Specific areas of interest include: Angiogenesis, growth and remodeling; Transport and exchange of gasses and solutes; Rheology and biorheology; Endothelial cell biology and metabolism; Interactions between endothelium, smooth muscle, parenchymal cells, leukocytes and platelets; Regulation of vasomotor tone; and Microvascular structures, imaging and morphometry. Papers also describe innovations in experimental techniques and instrumentation for studying all aspects of microcirculatory structure and function.
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