{"title":"铜弹DNA分析中回收金属离子的比较及铜、锌离子对DNA分析的影响","authors":"Natalia Czado, Rachel Houston, Sheree Hughes","doi":"10.1016/j.fsigss.2022.10.021","DOIUrl":null,"url":null,"abstract":"<div><p>Analyzing DNA from brass surfaces poses unique challenges that may result from DNA damage and/or PCR inhibition. To examine the relationship between the metal ions present in brass ammunition and the success of Short Tandem Repeat (STR) profiling, six recovery methods were tested to determine the identity and quantity of metal ions co-recovered during DNA sampling. In addition, DNA and metal ion solutions were created at varying concentrations to determine the threshold at which deleterious effects occur. The results of this study show that copper and zinc are recovered in the highest concentrations from both fired and unfired ammunition, but at substantially lower levels than previously published. Furthermore, most metal ions co-recovered with DNA were removed during DNA purification and complete STR profiles were generated when the concentrations of copper and zinc ions were less than 0.1 M and 0.03 M, respectively.</p></div>","PeriodicalId":56262,"journal":{"name":"Forensic Science International: Genetics Supplement Series","volume":"8 ","pages":"Pages 162-164"},"PeriodicalIF":0.5000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1875176822000592/pdfft?md5=5da3038e24447b8296fbabcd5f139d67&pid=1-s2.0-S1875176822000592-main.pdf","citationCount":"1","resultStr":"{\"title\":\"Comparison of metal ions recovered during DNA analysis of brass ammunition and effects of copper and zinc ions on DNA profiling\",\"authors\":\"Natalia Czado, Rachel Houston, Sheree Hughes\",\"doi\":\"10.1016/j.fsigss.2022.10.021\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Analyzing DNA from brass surfaces poses unique challenges that may result from DNA damage and/or PCR inhibition. To examine the relationship between the metal ions present in brass ammunition and the success of Short Tandem Repeat (STR) profiling, six recovery methods were tested to determine the identity and quantity of metal ions co-recovered during DNA sampling. In addition, DNA and metal ion solutions were created at varying concentrations to determine the threshold at which deleterious effects occur. The results of this study show that copper and zinc are recovered in the highest concentrations from both fired and unfired ammunition, but at substantially lower levels than previously published. Furthermore, most metal ions co-recovered with DNA were removed during DNA purification and complete STR profiles were generated when the concentrations of copper and zinc ions were less than 0.1 M and 0.03 M, respectively.</p></div>\",\"PeriodicalId\":56262,\"journal\":{\"name\":\"Forensic Science International: Genetics Supplement Series\",\"volume\":\"8 \",\"pages\":\"Pages 162-164\"},\"PeriodicalIF\":0.5000,\"publicationDate\":\"2022-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1875176822000592/pdfft?md5=5da3038e24447b8296fbabcd5f139d67&pid=1-s2.0-S1875176822000592-main.pdf\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Forensic Science International: Genetics Supplement Series\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1875176822000592\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Forensic Science International: Genetics Supplement Series","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1875176822000592","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
Comparison of metal ions recovered during DNA analysis of brass ammunition and effects of copper and zinc ions on DNA profiling
Analyzing DNA from brass surfaces poses unique challenges that may result from DNA damage and/or PCR inhibition. To examine the relationship between the metal ions present in brass ammunition and the success of Short Tandem Repeat (STR) profiling, six recovery methods were tested to determine the identity and quantity of metal ions co-recovered during DNA sampling. In addition, DNA and metal ion solutions were created at varying concentrations to determine the threshold at which deleterious effects occur. The results of this study show that copper and zinc are recovered in the highest concentrations from both fired and unfired ammunition, but at substantially lower levels than previously published. Furthermore, most metal ions co-recovered with DNA were removed during DNA purification and complete STR profiles were generated when the concentrations of copper and zinc ions were less than 0.1 M and 0.03 M, respectively.
期刊介绍：
The Journal of Forensic Science International Genetics Supplement Series is the perfect publication vehicle for the proceedings of a scientific symposium, commissioned thematic issues, or for disseminating a selection of invited articles. The Forensic Science International Genetics Supplement Series is part of a duo of publications on forensic genetics, published by Elsevier on behalf of the International Society for Forensic Genetics.
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