降解DNA的全基因组测序用于调查遗传谱系

IF 0.5 Q4 GENETICS & HEREDITY

Forensic Science International: Genetics Supplement Series Pub Date : 2022-12-01 DOI:10.1016/j.fsigss.2022.09.008

Janet Cady, Ellen M. Greytak

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引用次数: 2

摘要

全基因组测序为研究性遗传谱系学（IGG）分析不适合微阵列基因分型的具有挑战性的法医样本打开了大门。这些样本通常仍然没有达到足够高的覆盖率来进行直接基因型调用，因此使用1000基因组项目的数据评估了低覆盖率测序数据的插补管道。该管道生成的结果适用于低至0.25X覆盖范围的IGG。此外，对来自各种组织类型和输入量的法医样本进行了测序，并在插补后成功上传到遗传谱系数据库。

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Whole-genome sequencing of degraded DNA for investigative genetic genealogy

Whole genome sequencing has opened the doors to Investigative genetic genealogy (IGG) analysis of challenging forensic samples that are not suitable for microarray genotyping. These samples still do not typically achieve high enough coverage for direct genotype calling, therefore a pipeline for imputation from low coverage sequencing data was evaluated using data from the 1000 Genomes Project. This pipeline generated results suitable for IGG down to 0.25X coverage. Additionally, forensic samples from a variety of tissue types and input amounts were sequenced and successfully uploaded to genetic genealogy databases after imputation.

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来源期刊

Forensic Science International: Genetics Supplement Series Medicine-Pathology and Forensic Medicine

CiteScore

0.40

自引率

0.00%

发文量

122

审稿时长

25 days

期刊介绍： The Journal of Forensic Science International Genetics Supplement Series is the perfect publication vehicle for the proceedings of a scientific symposium, commissioned thematic issues, or for disseminating a selection of invited articles. The Forensic Science International Genetics Supplement Series is part of a duo of publications on forensic genetics, published by Elsevier on behalf of the International Society for Forensic Genetics.