{"title":"在聚合酶链反应中产生单链DNA的一种策略","authors":"Martim Kreitman , Laura F. Landweber","doi":"10.1016/0735-0651(89)90021-6","DOIUrl":null,"url":null,"abstract":"<div><p>A strategy for synthesizing single-stranded DNA by the polymerase chain reaction method is explored and two protocols are developed. DNA produced with these methods can be sequenced using standard <sup>35</sup>S or <sup>32</sup>P dideoxy sequencing protocols. The methods allow rapid genetic screening and population genetic analysis of sequence variation.</p></div>","PeriodicalId":77714,"journal":{"name":"Gene analysis techniques","volume":"6 4","pages":"Pages 84-88"},"PeriodicalIF":0.0000,"publicationDate":"1989-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0735-0651(89)90021-6","citationCount":"50","resultStr":"{\"title\":\"A strategy for producing single-stranded DNA in the polymerase chain reaction\",\"authors\":\"Martim Kreitman , Laura F. Landweber\",\"doi\":\"10.1016/0735-0651(89)90021-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A strategy for synthesizing single-stranded DNA by the polymerase chain reaction method is explored and two protocols are developed. DNA produced with these methods can be sequenced using standard <sup>35</sup>S or <sup>32</sup>P dideoxy sequencing protocols. The methods allow rapid genetic screening and population genetic analysis of sequence variation.</p></div>\",\"PeriodicalId\":77714,\"journal\":{\"name\":\"Gene analysis techniques\",\"volume\":\"6 4\",\"pages\":\"Pages 84-88\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1989-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0735-0651(89)90021-6\",\"citationCount\":\"50\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gene analysis techniques\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0735065189900216\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene analysis techniques","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0735065189900216","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A strategy for producing single-stranded DNA in the polymerase chain reaction
A strategy for synthesizing single-stranded DNA by the polymerase chain reaction method is explored and two protocols are developed. DNA produced with these methods can be sequenced using standard 35S or 32P dideoxy sequencing protocols. The methods allow rapid genetic screening and population genetic analysis of sequence variation.
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