缺陷大肠杆菌β-d-半乳糖苷酶免疫激活的分子机制

Everly Conway De Macario, Alberto J.L. Macario
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引用次数: 1

摘要

大肠杆菌β-d-半乳糖苷酶抗体形成不同的亚群,在反应过程中独立分离:从而区分沉淀分子和激活分子。后一种分子与有缺陷的突变酶发生反应。AMEF,并激活其催化位点,然后与亚硫酸酯相互作用。作为阐明AMEF激活机制的一步,测定了相关抗酶抗体的一些特性。抗体种类和效价不影响激活:在AMEF浓度的100倍范围内,IgM、IgG和Fab分子获得了相同的激活。沉淀的抗体不影响活化或与活化分子竞争。15只家兔产生的抗酶抗体大部分或全部为IgG。三只兔子也产生了少量的IgM抗体,但仅在免疫后2周内产生。来自免疫血清的非活化IgM分子不与活化IgG分子竞争,无论这些IgM分子是否能够结合酶,如沉淀或与抗μ链抗血清共沉淀所证明的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular mechanisms of the immunologic activation of a defectiveescherichia coli β-d-galactosidase—I

Antibodies toEscherichia coli β-d-galactosidase form distinct sub-populations which segregate independently during the response: thus precipitating and activating molecules are distinguished. The latter molecules react with a defective mutant enzyme. AMEF, and activate its catalytic site, which then interacts with the subsrate. As a step toward elucidating the mechanism of AMEF activation, some properties of the relevant anti-enzyme antibodies were determined. Antibody class and valence do not affect activation: equal activation was obtained with IgM, IgG and Fab molecules over a 100-fold range of AMEF concentrations. Precipitating antibodies do not influence activation or compete with activating molecules. Most or all antienzyme antibodies produced by 15 rabbits were IgG. Three rabbits also produced small amounts of IgM antibodies, but only within 2 weeks after immunization. Non-activating IgM molecules from immune sera did not compete with activating IgG molecules, whether or not these IgM molecules were able to bind the enzyme, as demonstrated by precipitation or by coprecipitation with anti-μ-chain antisera.

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