Llanos Salar-Vidal , Catarina Chaves , Ileana T. Dianzo-Delgado , Patricio Favier , Salvador Giner-Almaraz , María José Gómez-Gómez , Guillermo Martín-Gutiérrez , Isabel Pereira , Ana Rodríguez-Fernández , Patricia Ruiz-Garbajosa , Carlos Salas-Venero , Jaime Esteban
{"title":"BioFire JI小组的多中心评估与改善急性骨关节感染的微生物学诊断有关。","authors":"Llanos Salar-Vidal , Catarina Chaves , Ileana T. Dianzo-Delgado , Patricio Favier , Salvador Giner-Almaraz , María José Gómez-Gómez , Guillermo Martín-Gutiérrez , Isabel Pereira , Ana Rodríguez-Fernández , Patricia Ruiz-Garbajosa , Carlos Salas-Venero , Jaime Esteban","doi":"10.1016/j.ijmm.2023.151588","DOIUrl":null,"url":null,"abstract":"<div><p>Microbiological diagnosis of osteoarticular infections (OI) is crucial for a successful treatment. A prospective multicenter study including 262 synovial fluids with suspicion of acute OI was performed between July 2021 and October of 2022. BioFire Joint Infection Panel multiplex-PCR test was performed and results were compared with conventional cultures of synovial fluid specimens. In total, 136 microorganisms were detected, and fourteen samples were positive for more than one microorganism. In monomicrobial infections (n = 87) agreement with culture was 69%. In 26 samples, the multiplex PCR yield an additional positive result when culture result was negative. It helped in the detection of fastidious microorganisms as <em>K. kingae</em> and <em>N. gonorrhoeae</em>. This multiplex PCR has proven to be a useful technique that can be used for patients with high suspicion of acute OI in a rapid and automated manner.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 6","pages":"Article 151588"},"PeriodicalIF":4.5000,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Multicenter evaluation of BioFire JI panel related to improved microbiological diagnostics on acute osteoarticular infections\",\"authors\":\"Llanos Salar-Vidal , Catarina Chaves , Ileana T. Dianzo-Delgado , Patricio Favier , Salvador Giner-Almaraz , María José Gómez-Gómez , Guillermo Martín-Gutiérrez , Isabel Pereira , Ana Rodríguez-Fernández , Patricia Ruiz-Garbajosa , Carlos Salas-Venero , Jaime Esteban\",\"doi\":\"10.1016/j.ijmm.2023.151588\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Microbiological diagnosis of osteoarticular infections (OI) is crucial for a successful treatment. A prospective multicenter study including 262 synovial fluids with suspicion of acute OI was performed between July 2021 and October of 2022. BioFire Joint Infection Panel multiplex-PCR test was performed and results were compared with conventional cultures of synovial fluid specimens. In total, 136 microorganisms were detected, and fourteen samples were positive for more than one microorganism. In monomicrobial infections (n = 87) agreement with culture was 69%. In 26 samples, the multiplex PCR yield an additional positive result when culture result was negative. It helped in the detection of fastidious microorganisms as <em>K. kingae</em> and <em>N. gonorrhoeae</em>. This multiplex PCR has proven to be a useful technique that can be used for patients with high suspicion of acute OI in a rapid and automated manner.</p></div>\",\"PeriodicalId\":50312,\"journal\":{\"name\":\"International Journal of Medical Microbiology\",\"volume\":\"313 6\",\"pages\":\"Article 151588\"},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2023-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Medical Microbiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1438422123000164\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Medical Microbiology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1438422123000164","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Multicenter evaluation of BioFire JI panel related to improved microbiological diagnostics on acute osteoarticular infections
Microbiological diagnosis of osteoarticular infections (OI) is crucial for a successful treatment. A prospective multicenter study including 262 synovial fluids with suspicion of acute OI was performed between July 2021 and October of 2022. BioFire Joint Infection Panel multiplex-PCR test was performed and results were compared with conventional cultures of synovial fluid specimens. In total, 136 microorganisms were detected, and fourteen samples were positive for more than one microorganism. In monomicrobial infections (n = 87) agreement with culture was 69%. In 26 samples, the multiplex PCR yield an additional positive result when culture result was negative. It helped in the detection of fastidious microorganisms as K. kingae and N. gonorrhoeae. This multiplex PCR has proven to be a useful technique that can be used for patients with high suspicion of acute OI in a rapid and automated manner.
期刊介绍：
Pathogen genome sequencing projects have provided a wealth of data that need to be set in context to pathogenicity and the outcome of infections. In addition, the interplay between a pathogen and its host cell has become increasingly important to understand and interfere with diseases caused by microbial pathogens. IJMM meets these needs by focussing on genome and proteome analyses, studies dealing with the molecular mechanisms of pathogenicity and the evolution of pathogenic agents, the interactions between pathogens and host cells ("cellular microbiology"), and molecular epidemiology. To help the reader keeping up with the rapidly evolving new findings in the field of medical microbiology, IJMM publishes original articles, case studies and topical, state-of-the-art mini-reviews in a well balanced fashion. All articles are strictly peer-reviewed. Important topics are reinforced by 2 special issues per year dedicated to a particular theme. Finally, at irregular intervals, current opinions on recent or future developments in medical microbiology are presented in an editorial section.
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