pH值对人RPA DNA结合亲和力影响的核磁共振研究

IF 0.4 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of the Korean magnetic resonance society Pub Date : 2016-09-20 DOI:10.6564/JKMRS.2016.20.3.071

Min-Woo Lee, Jungyuen Choi, Jae-Gyu Choi, Ae-Ree Lee, Joon-Hwa Lee

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引用次数: 0

摘要

复制蛋白A (RPA)在DNA复制、重组和修复中起着至关重要的作用。RPA由70、32和14 kDa亚基组成，具有较高的单链DNA (ssDNA)结合亲和力。最大的亚基RPA70主要与ssDNA结合，并与许多细胞和病毒蛋白相互作用。在本研究中，我们对人类RPA70的DNA结合域A (RPA70A)与ssDNA, d(CCCCC)在不同pH下的复合物进行核磁共振实验，了解pH对RPA70A的ssDNA结合的影响。结合残基的化学位移扰动在pH 6.5时最为显著，随pH的增加而减小。本研究对ssDNA结合人RPA的分子机制提供了有价值的见解。

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NMR Study of the pH Effect on the DNA Binding Affinity of Human RPA

The replication protein A (RPA) plays a crucial role in DNA replication, recombination, and repair. RPA consists of 70, 32 and 14 kDa subunits and has high single-stranded DNA (ssDNA) binding affinity. The largest subunit, RPA70, mainly contributes to bind to ssDNA as well as interact with many cellular and viral proteins. In this study, we performed nuclear magnetic resonance experiments on the complex of the DNA binding domain A of human RPA70 (RPA70A) with ssDNA, d(CCCCC), at various pH, to understand the effect of pH on the ssDNA binding of RPA70A. The chemical shift perturbations of binding residues were most significant at pH 6.5 and they reduced with pH increment. This study provides valuable insights into the molecular mechanism of the ssDNA binding of human RPA.

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来源期刊

Journal of the Korean magnetic resonance society BIOCHEMICAL RESEARCH METHODS-

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66.70%

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