印度北卡纳塔克邦三级医院登革热血清流行趋势

A. Parande, Rupapali Shinde, M. Parande, Rubeena Ishrat Mulla, Uma Chikkaraddi
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引用次数: 1

摘要

简介:黄病毒科的登革病毒是登革热的病原体。埃及伊蚊是其主要传播媒介。虽然登革热病例较轻且可自行消退,但可能出现致命的并发症,如登革出血热和登革休克综合征。目的:探讨登革热在疑似病例血清中的流行趋势。材料与方法:本研究是一项横断面研究,于2017年1月至2019年12月在印度卡纳塔克邦Belagavi医学科学研究所(BIMS)进行。采集疑似登革热病例血清样本,采用免疫球蛋白M (IgM)捕获酶联免疫吸附试验(ELISA)检测登革热病毒IgM抗体,采用浦那国家病毒学研究所(NIV)生产的ELISA试剂盒检测登革热非结构蛋白1抗原NS1捕获ELISA。这些测试是按照制造商的说明进行的。从研究中获得的数据使用描述性统计进行分析。结果:三年间共检测了8992份血清样本,其中1340份(14.90%)为登革热感染阳性。其中抗登革热IgM抗体阳性1048例(78.21%),NS1抗原阳性109例(8.13%),两者均阳性183例(13.66%)。发病年龄以11-20岁为主,男女比例为1.18:1。季节高峰为季风,即6月(15.52%)次之,8月(12.02%)。结论:登革热感染的血清阳性率是关键,提示IgM抗体和NS1抗原的检测对登革热感染的诊断具有重要意义。该研究还确定了年轻人群的风险更高,季风是该地区病毒传播的最有利季节,并强调了协调一致努力控制和预防疾病的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Trends in the Seroprevalence of Dengue in a Tertiary Care Hospital of North Karnataka, India
Introduction: Dengue virus of the Flaviviridae family is the causative agent of dengue fever. The Aedes aegypti mosquito is the main vector for its transmission. Though, the cases of dengue fever are mild and self-resolving, there can be fatal complications like Dengue Haemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS). Aim: To study the trends in the seroprevalence of dengue in serum samples of suspected cases. Materials and Methods: The present study was a cross- sectional study which was conducted from January 2017 to December 2019, at Belagavi Institute of Medical Sciences (BIMS), Belagavi, Karnataka, India. The serum samples were collected from suspected dengue fever cases and tested by Immunoglobulin M (IgM) capture Enzyme Linked Immunosorbent Assay (ELISA), to detect IgM antibody against dengue virus and NS1 capture ELISA for dengue NS1 (nonstructural protein 1) antigen using ELISA kits manufactured by National Institute of Virology (NIV), Pune. The tests were performed according to the manufacturer’s instruction. The data obtained from the study was analysed using descriptive statistics. Results: A total of 8,992 serum samples were tested over a period of three years, of which 1,340 (14.90%) were positive for dengue infection. Among which 1,048 (78.21%) were positive for anti-dengue IgM antibodies, 109 (8.13%) were positive for NS1 antigen and 183 (13.66%) were positive for both. Most affected age group was 11-20 years and male to female ratio is 1.18:1. The seasonal peak was observed in monsoon i.e. month of June (15.52%) followed by August (12.02%). Conclusion: Seroprevalence of dengue infection being critical signifies the importance of detection of both IgM antibodies and NS1 antigen for diagnosis of dengue infection. The study also identifies younger population being at higher risk and also monsoon as the most favourable season for viral transmission in this region and highlights the importance of concerted efforts towards disease control and prevention.
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