湿片和细胞自旋涂片在诊断尿路上皮癌中的作用:一项描述性观察研究

A. Patil, P. Shashikala, P. M. Pattar, K. Arpitha
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引用次数: 0

摘要

导读:下尿路最常见的癌为尿路上皮型,多数发生在膀胱。尿沉积物细胞学检查显示脱落的肿瘤细胞是研究的选择之一。目的:研究未染色湿片制剂和巴氏染色细胞自旋涂片获得的尿沉渣的非典型细胞形态。材料和方法:这是一项描述性观察性研究,为期29个月,即2016年7月至2018年12月,在印度卡纳塔克邦达文杰尔SS医学科学与研究中心进行。采用非概率抽样方法,用dirui - h500型半自动尿液分析仪对50份尿样进行分析,并采用湿片制备和细胞自旋涂片法进行巴氏染色。根据细胞学和细胞核的细节来分析涂片对非典型细胞的阳性或阴性。结果:细胞自旋制剂能显著提高红细胞的产量,且红细胞在湿片上的形态比细胞自旋制剂更清晰。细胞自旋制备的非典型细胞产量显著增加,pap染色的细胞自旋制备的非典型细胞的形态比湿贴尿检查的更清晰。结论:总体而言,细胞自纺丝法对白细胞、上皮细胞和非典型细胞形态的细胞产量和保存效果较好,湿载法对红细胞的保存效果较好。细胞自旋技术是一种快速、高效和经济的方法,可以在较少的细胞样品中提高细胞产量,也有助于提供更好的细胞形态学细节。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of Wet Mount and Cytospin Smears in Diagnosing Urothelial Carcinoma: A Descriptive Observational Study
Introduction: Most common carcinomas of lower urinary tract are of urothelial type in which, majority of them occur in the bladder. The demonstration of exfoliated neoplastic cells in urine sediment cytology is one of the choices of investigation. Aim: To study the morphology of atypical cells of urine sediment in unstained wet mount preparation and urine sediment obtained by cytospin smear stained with Papanicolaou stain. Materials and Methods: It was a descriptive observational study done over a period of 29 months i.e., from July 2016 to December 2018 at SS Institute of Medical Sciences and Research Centre, Davangere, Karnataka, India. Using non probability sampling method, 50 urine samples were analysed by DIRUI-H 500 a semi automatic urine analyser, which were further examined by wet mount preparation and cytospin smear stained with papanicolaou stain. Results were analysed by whether the smears were positive or negative for atypical cells based on the cytological and nuclear details. Results: The yield of Red Blood Corpuscles (RBCs) was significantly increased in cytospin preparation with morphology of RBCs was better appreciated in wet mount compared to cytospin preparation. The yield of atypical cells was significantly increased in cytospin preparation with the morphology of atypical cells were better appreciated in pap stained cytospin preparation compared to wet mount urine examination. Conclusion: Overall, cell yield and preservation of White Blood Corpuscles (WBCs), epithelial cell and atypical cells morphology was better in cytospin preparation, while RBCs were better appreciated in wet mount preparation. Cytospin technology is a quick, efficient and cost effective method for increasing cell yield in less cellular samples and also helps in providing better cellular morphological details.
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