印度古吉拉特邦单峰骆驼伊文氏锥虫的寄生虫学和分子发病率

Q4 Agricultural and Biological Sciences

Journal of Camel Practice and Research Pub Date : 2022-01-01 DOI:10.5958/2277-8934.2022.00022.4

B. Thakre, B. Kumar, Nilima N. Brahmbhatt, K. Gamit

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引用次数: 0

摘要

本研究旨在通过显微镜和聚合酶链反应(PCR)方法评估印度古吉拉特邦Saurashtra地区骆驼中伊文氏弓形虫感染的发生率。随机采集了192头骆驼的血液样本。评价Field染色法、Giemsa染色法和RoTat1.2聚合酶链反应(PCR)法检测血样中伊文氏弓形虫的诊断敏感性和特异性。Field’s染色法、Giemsa染色法和RoTat1.2 PCR法记录的骆驼伊文氏体感染率分别为3.23%、8.85%和22.39%。与吉姆萨染色法相比，Field染色法检测伊文氏弓形虫的灵敏度为35.29%，PCR法检测伊文氏弓形虫的灵敏度为100%。新开发的RoTat1.2 PCR检测的分析灵敏度为1.07 pg纯化全血基因组DNA。在流行病学上，伊文氏体是各年龄组骆驼的重要病原体，但在成虫和季风季节更为流行。

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Parasitological and Molecular Incidence of Trypanosoma Evansi in Dromedary Camels of Gujarat, India

The present study was aimed to assess the incidence of T. evansi infections in camel in Saurashtra region of Gujarat, India, through microscopy and polymerase chain reaction (PCR) methods. Blood samples were collected from 192 camels, randomly. The diagnostic sensitivity and specificity of Field’s stain, Giemsa stain and RoTat1.2 polymerase chain reaction (PCR) assays for detection of T. evansi in blood samples was evaluated. T. evansi infection recorded in camels based on Field’s stain, Giemsa stain and RoTat1.2 PCR assays was 3.23%, 8.85% and 22.39%, respectively. Sensitivity of Field’s stain was 35.29% while PCR test had 100% sensitivity in comparison to Giemsa stain for the detection of T. evansi in blood samples. The analytical sensitivity of newly developed RoTat1.2 PCR assay was determined as 1.07 pg of purified whole blood genomic DNA. Epidemiologically, T. evansi was an important pathogen in camels of all age groups but more prevalent in adult animals and in monsoon season.

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来源期刊

Journal of Camel Practice and Research 农林科学-兽医学

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： JCPR is an exclusive journal which brings out the manuscripts based on New World and Old World camelids. This journal provided a very good platform to publish camelid literature with a view to find the missing links of research and to update the camelids practitioners and researchers with latest research.