em和γ射线处理分离鹰嘴豆突变体的分子谱分析

Q4 Biochemistry, Genetics and Molecular Biology
S. Umavathi, L. Mullainathan
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引用次数: 0

摘要

传统的突变育种技术经常被用来提高作物的产量和抗病虫害能力。以泰米尔纳德邦农业大学获得的鹰嘴豆Co -4为材料,采用两种著名诱变剂甲烷磺酸乙酯和EMS诱变。用不同浓度的EMS (10 ~ 50 mM)和γ射线(20 ~ 60 kR)处理种子。共分离和鉴定了369个突变体,这些突变体改变了植物的高度、叶结构、花的颜色、种子、荚果大小和持续时间。其中,选择直接影响作物经济价值的5个真育种突变体,即早花突变体(EFM)、果荚突变体(BPM)、果籽突变体(BSM)、高产突变体(HYM)和高蛋白含量突变体(HPC)进行进一步研究。结果表明,各突变系间的质量性状均有显著的正增加。分离突变体的RAPD图谱共发现129个条带,其中92个条带为多态,平均多态性率为70.99%。除28条条带外,其余条带均为突变。新条带的出现和现有条带的缺失可能是鹰嘴豆品质改善的原因。RAPD被认为是区分突变体与其亲本、评估遗传变异和系统发育关系的良好标记。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular profiling of chickpea mutants isolated from EMS and gamma rays treatments
Conventional mutation breeding techniques have often been used to improve yield, disease and pest resistance in crop plants. In the present attempt, two well-known mutagenic agents ethyl methane sulphonate and EMS were employed in order to induce genetic variability variety of chick pea Co -4 obtained from Tamil Nadu Agricultural University. The seeds were treated with different concentrations of EMS (10-50 mM) and Gamma rays (20-60 kR). A total of 369 mutants which alter the height, leaf structure, colour of flower, seed, size of pod, duration of the plant were isolated and characterized. Among them, five true breeding mutants namely Early flowering mutant (EFM), Bold pod mutant (BPM), Bold seed mutant BSM), High yielding mutant (HYM) and High protein content mutant (HPC), which directly influenced the economic value of the crop were selected for further study. The results revealed a significant positive increase in the qualitative traits among the mutant lines. The RAPD profiling of isolated mutants revealed a total of 129 bands, among them 92 were polymorphic with an average of 70.99 percentage of polymorphism. Apart from 28 bands, all the bands seemed to be mutation. The presence of new bands and absence of existing bands might be the reason for quality improvement of chickpea. The RAPD is considered as an excellent marker to differentiate mutants from their parents, to assess genetic variation and phylogenetic relationship.
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来源期刊
Ratarstvo i Povrtarstvo
Ratarstvo i Povrtarstvo Agricultural and Biological Sciences-Soil Science
CiteScore
0.80
自引率
0.00%
发文量
4
审稿时长
4 weeks
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