活的和灭活的肠炎沙门氏菌疫苗:肉鸡种鸡的免疫机制

Letícia Dal Bérto, B. Beirão, T. F. Filho, M. Ingberman, Celso Fávaro, R. Tavella, Rebeca Buest de Mesquita Silva, L. Caron
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引用次数: 9

摘要

沙门氏菌是一种普遍存在的病原体,除了引起家禽疾病外,还具有日益增长的人畜共患影响。它要求实施多种控制战略,其中疫苗发挥重要作用。了解不同疫苗引发的免疫途径是很重要的,例如,有助于建立保护的强免疫相关性。为了确定疫苗接种对肉鸡体液和细胞免疫反应的动力学,用肠炎沙门氏菌活疫苗或灭活疫苗对肉鸡(Cobb Slow)进行了免疫。流式细胞术分析外周血淋巴细胞和巨噬细胞亚群,定量抗原特异性循环IgY和粘膜IgA。流式细胞术检测的标志物为CD8/CD28、CD4/TCRVβ1、Kul/ MHC II和Bu-1。与未接种疫苗的对照组相比,活疫苗和灭活疫苗在某些时间点诱导循环单核细胞(Kul+MHCII+)的比例增加。然而,与对照组相比,活疫苗导致CD8-CD28+和Bu-1+淋巴细胞的增加,而灭活疫苗在增强剂量后导致几种白细胞亚群(kl - mhcii +, Bu-1+, CD8+CD28+, CD8-CD28+, CD4+TCRVβ1-, CD4+TCRVβ1+, CD4+TCRVβ1+)的百分比降低。两种疫苗均诱导特异性血清IgY和黏膜IgA产生;然而,灭活疫苗在较短的时间内刺激了较高的滴度。这些结果有助于了解沙门氏菌活疫苗和灭活疫苗对鸡的作用机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Live and Inactivated Salmonella Enteritidis Vaccines: Immune Mechanisms in Broiler Breeders
Salmonella is a ubiquitous pathogen which, in addition to causing poultry diseases, has a growing zoonotic impact. It has demanded the implementation of diverse control strategies, in which vaccines play a major role. The understanding of the immune pathways elicited by the different vaccines is important, contributing for the establishment of strong immune correlates of protection, for instance. With the purpose of determining the dynamics of the humoral and cellular immune responses to vaccination, broiler breeders (Cobb Slow) were immunized with live or inactivated vaccines against Salmonella Enteritidis. Lymphocyte and macrophage subsets were analyzed in the peripheral blood by flow cytometry and antigen-specific circulating IgY and mucosal IgA were quantified. The markers analyzed by flow cytometry were CD8/CD28, CD4/TCRVβ1, Kul/ MHC II and Bu-1. Both live and inactivated vaccines induced an increase in the proportion of circulating monocytes (Kul+MHCII+) in some time points compared to non-vaccinated controls. However, whereas the live vaccine leads to an increase in CD8-CD28+ and Bu-1+ lymphocytescompared to the control group, the inactivated vaccine prompteda reduction in the percentage of severalleucocyte subsets (Kul-MHCII+, Bu-1+, CD8+CD28+, CD8-CD28+, CD4+TCRVβ1-, CD4+TCRVβ1+, CD4-TCRVβ1+) after the boost dose. Both vaccines induced specific serum IgY and mucosal IgA production; however, the inactivated vaccine stimulated higher titers in a shorter period. These results contribute to the understanding of mechanisms of action of live and inactivated Salmonella vaccines in chickens.
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